Fast Electron Transfer from Cytochrome c 6 and Plastocyanin to Photosystem I of Chlamydomonas reinhardtii Requires PsaF
1997; American Chemical Society; Volume: 36; Issue: 21 Linguagem: Inglês
10.1021/bi970082c
ISSN1943-295X
AutoresMichael Hippler, Friedel Drepper, Joseph A Farah, Jean‐David Rochaix,
Tópico(s)Spectroscopy and Quantum Chemical Studies
ResumoTo study the function of the PsaF subunit of photosystem I (PSI), the interactions between plastocyanin or cytochrome c6 and PSI isolated from wild-type and a PsaF-deficient mutant of Chlamydomonas reinhardtii have been examined using cross-linking techniques and flash absorption spectroscopy. We show that efficient electron transfer from both plastocyanin and cytochrome c6 to PSI depends on PsaF. A remarkable feature of the PSI complex of C. reinhardtii is that both plastocyanin and cytochrome c6 reduce P700+ with first-order kinetics and a half-time of 3 μs, which is unique among photosynthetic organisms examined.
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