High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture
2007; Elsevier BV; Volume: 41; Issue: 3 Linguagem: Inglês
10.1016/j.enzmictec.2007.02.008
ISSN1879-0909
AutoresYonghong Li, Zongbao K. Zhao, Feng‐Wu Bai,
Tópico(s)Biofuel production and bioconversion
ResumoMicrobial lipid production by the oleaginous yeast Rhorosporidium toruloides Y4 was studied using glucose as carbon source, in order to realize high-density cell culture. Batch cultures demonstrated that there was little inhibitory effect with a substrate concentration of up to 150 g l−1. Flask fed-batch cultures were run for 25 days and reached a dry biomass and cellular lipid content of 151.5 g l−1 and 48.0% (w/w), respectively. Using pilot-scale fed-batch cultures in a 15-l stirred-tank fermenter for 134 h resulted in dry biomass, lipid content and lipid productivity of 106.5 g l−1, 67.5% (w/w) and 0.54 g l−1 h−1, respectively. The fed-batch culture model used here featured initial nutrient-rich media and a pure carbon source with discontinuous feeding. Gas chromatography analysis revealed that lipids from R. toruloides Y4 contained mainly long-chain fatty acids with 16 and 18 carbon atoms. The four major constituent fatty acids were oleic acid, palmitic acid, stearic acid and linoleic acid. A slight increase in stearic acid production was found during the culture process. Based on these compositional data, microbial lipids from R. toruloides Y4 are a potential alternative oil resource for biodiesel production.
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