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TLC Blot (Far-Eastern Blot) and Its Applications

2009; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-59745-542-8_55

1940-6029

Takao Taki, Tania Valdes Gonzalez, Naoko Goto‐Inoue, Takahiro Hayasaka, Mitsutoshi Setou,

Metabolism and Genetic Disorders

A simple method for transfer of lipids including phospholipids, glycolipids, and neutral lipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane, called TLC blot (far-eastern blot), is presented. Lipids separated on a HPTLC plate are blotted quantitatively. This procedure made it possible to purify individual lipids from a blotted membrane in a short time. Binding study, immunodetection, and mass spectrometric analysis are available for PVDF membrane. Furthermore, the world of molecular species imaging is opened by a scanning analysis with a combination of TLC blot and matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometer (TLC-Blot/MALDI-TOF MS).