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Cryopreservation of Sperm: Indications, Methods and Results

2003; Lippincott Williams & Wilkins; Volume: 170; Issue: 4 Part 1 Linguagem: Inglês

10.1097/01.ju.0000084820.98430.b8

1527-3792

Jennifer T. Anger, Bruce R. Gilbert, Marc Goldstein,

Reproductive Biology and Fertility

No AccessJournal of UrologyCLINICAL UROLOGY: Review Articles1 Oct 2003Cryopreservation of Sperm: Indications, Methods and Results JENNIFER TASH ANGER, BRUCE R. GILBERT, and MARC GOLDSTEIN JENNIFER TASH ANGERJENNIFER TASH ANGER , BRUCE R. GILBERTBRUCE R. GILBERT , and MARC GOLDSTEINMARC GOLDSTEIN View All Author Informationhttps://doi.org/10.1097/01.ju.0000084820.98430.b8AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We review the history of the cryopreservation of human sperm. The current methods of cryopreservation and indications are highlighted, and the success rates of the various uses of cryopreserved sperm are reviewed. Potential adverse sequelae of human cryopreservation are also discussed. Materials and Methods: Indications for cryopreservation include several applications, namely homologous and donor insemination, cryopreservation prior to surgical infertility treatment, intraoperative cryopreservation, postoperative cryopreservation, cryopreservation prior to treatment for malignancies and nonmalignant diseases, as well as premortem and postmortem cryopreservation. Results: With the use of intracytoplasmic sperm injection, open microsurgical epididymal sperm aspiration and testicular sperm extraction with cryopreserved sperm in men with obstructive azoospermia yields pregnancy rates similar to those using fresh sperm. Intraoperative cryopreservation is recommended for complex reconstructive cases with lower patency rates, including vasoepididymostomy and vasoepididymostomy to the efferent ductule. In addition, sperm banking is strongly recommended for all patients with malignant disease who may wish to have children, since a small but significant percent of these men later use their banked semen. Although there is concern about the transfer of genetically damaged sperm after cryopreservation, no proven increase in genetic or phenotypic abnormalities in offspring has been identified. Conclusions: Sperm cryopreservation has revolutionized the field of assisted reproduction. Cryopreserving sperm avoids the need for additional surgery in couples undergoing repeated in vitro fertilization/intracytoplasmic sperm injection cycles. Moreover, it provides hope for men undergoing chemotherapy, radiation or radical surgery who once had no chance for future fertility. References 1 : Role of spermatozoa cryopreservation in assisted reproduction. In: Human Spermatozoa in Assisted Reproduction. Edited by . Baltimore: Williams & Wilkins Co.1990: 100. chapt. 10. 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Google Scholar From the James Buchanan Brady Urology Foundation, Department of Urology and Center for Male Reproductive Medicine and Microsurgery, Cornell Institute for Reproductive Medicine New York-Presbyterian Hospital, Weill Medical College of Cornell University, New York, New York© 2003 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 170Issue 4 Part 1October 2003Page: 1079-1084 Advertisement Copyright & Permissions© 2003 by American Urological Association, Inc.Keywordssperm preservationspermcryopreservationtestisinfertility, maleMetricsAuthor Information JENNIFER TASH ANGER More articles by this author BRUCE R. GILBERT More articles by this author MARC GOLDSTEIN More articles by this author Expand All Advertisement PDF downloadLoading ...