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Psychromonas ingrahamii sp. nov., a novel gas vacuolate, psychrophilic bacterium isolated from Arctic polar sea ice

2006; Microbiology Society; Volume: 56; Issue: 5 Linguagem: Inglês

10.1099/ijs.0.64068-0

1466-5034

Ann J. Auman, Jennifer L. Breezee, John J. Gosink, Peter Kämpfer, James T. Staley,

Genomics and Phylogenetic Studies

A gas vacuolate bacterium, designated strain 37 T , was isolated from a sea ice core collected from Point Barrow, Alaska, USA. Cells of strain 37 T were large (6–14 μm in length), rod-shaped, contained gas vacuoles of two distinct morphologies, and grew well at NaCl concentrations of 1–10 % and at temperatures of −12 to 10 °C. The DNA G+C content was 40 mol%. Whole-cell fatty acid analysis showed that 16 : 1 ω 7 c comprised 67 % of the total fatty acid content. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus Psychromonas , with highest sequence similarity (96.8 %) to Psychromonas antarctica . Phenotypic analysis differentiated strain 37 T from P. antarctica on the basis of several characteristics, including cell morphology, growth temperature range and the ability to hydrolyse polymers. DNA–DNA hybridization experiments revealed a level of relatedness of 37 % between strain 37 T and P. antarctica , providing further support that it represents a distinct species. The name Psychromonas ingrahamii sp. nov. is proposed for this novel species. The type strain is 37 T (=CCUG 51855 T =CIP 108865 T ).