Acoustic microscopy of human metaphase chromosomes
1980; Wiley; Volume: 120; Issue: 2 Linguagem: Inglês
10.1111/j.1365-2818.1980.tb04135.x
ISSN1365-2818
AutoresD. Rugar, Joseph E. Heiserman, Shelley K. Minden, C. F. Quate,
Tópico(s)RNA and protein synthesis mechanisms
ResumoSUMMARY Acoustic micrographs of human metaphase chromosomes have been recorded with wavelengths as short as 470 nm using liquid argon near 85 K as the acoustic wave coupling medium. Chromosomes prepared by trypsin‐Giemsa staining exhibit acoustic banding patterns similar to the G‐bands seen in optical images. Unstained chromosomes exhibit acoustic markings that do not correspond to traditional banding patterns. The observed acoustic contrast may arise from spatial variations in chromosomal mechanical properties, or from thickness variations in the fixed chromosomes.
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