Antioxidants protect against reactive oxygen species associated with adriamycin-treated cardiomyocytes
1999; Elsevier BV; Volume: 136; Issue: 1 Linguagem: Inglês
10.1016/s0304-3835(98)00306-1
ISSN1872-7980
AutoresS.Michael DeAtley, Michael Y. Aksenov, Marina Aksenova, Bart Harris, R. W. Hadley, Pamela Cole Harper, John M. Carney, D. Allan Butterfield,
Tópico(s)Coenzyme Q10 studies and effects
ResumoAdriamycin (ADM) is a broad-spectrum antineoplastic antibiotic used to treat cancer patients. However, the usefulness of this drug is presently limited by the development of a dose-dependent cardiotoxicity. A current hypothesis for the ADM-induced cardiotoxicity is the production of reactive oxygen radicals by the drug. We utilized the fluorescent indicator 2′,7′-dichlorodihydrofluorescein diacetate (DCFH/DA), in which fluorescence appears if reactive oxygen species (ROS) are present, to investigate the ability of ADM to generate reactive oxygen species and the potential protective effect of antioxidants in a cultured cardiomyocyte model. All three of the antioxidants (alpha-phenyl-tert-butyl nitrone (PBN), trolox, and 5-aminosalicyclic acid (5-ASA)) tested in our ADM-treated myocytes provided protection against the oxidative stress induced by the drug. These findings suggest that antioxidants modulate ADM-induced oxidative stress, and they are discussed in terms of a possible therapeutic strategy in the prevention of cardiotoxicity resulting from ADM administration.
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