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Effect of mitomycin C on the neural tube defects of the curly‐tail mouse

1986; Wiley; Volume: 33; Issue: 3 Linguagem: Inglês

10.1002/tera.1420330308

2472-1727

Mary J. Seller, Kathleen J. Perkins,

Spinal Dysraphism and Malformations

Abstract Around 60% of the mouse mutants called curly‐tail, have tail aberrations in the form of a coil or a kink, with or without lumbosacral spina bifida, and rarely, exencephaly. These neural tube defects (NTD) are the result of an incompletely penetrant recessive gene. A single injection of various doses (1–6 mg/kg) of the DNA inhibitor mitomycin C was given to pregnant curlytail mice on day 7, 8, or 9 of gestation, and its effect on the NTD of the embryos was noted. No dose used was lethal to the embryo. When given on day 7 or day 8, mitomycin C markedly increased the number of exencephalics, and additionally, on day 8, it reduced the number of posterior abnormalities. However, on day 9, no exencephaly was produced, and there was a drastic reduction in the number of tail and spinal defects, the overall incidence of NTD being as low as 15% with 2 mg/kg. A twofold effect of mitomycin C on the curly‐tail embryos was thus observed–according to the time in development it was administered, firstly, a teratogenic effect, and later, a “remedial” or preventive effect.