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Sphingomonas jaspsi sp. nov., a novel carotenoid-producing bacterium isolated from Misasa, Tottori, Japan

2007; Microbiology Society; Volume: 57; Issue: 7 Linguagem: Inglês

10.1099/ijs.0.64828-0

1466-5034

Dalal Asker, Teruhiko Beppu, Kenji Uéda,

Plant and Fungal Species Descriptions

A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16 T ) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16 T was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells) −1 ]. The DNA G+C content of strain TDMA-16 T was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16 T in a distinct lineage in the family Sphingomonadaceae ; sequence data showed that strain TDMA-16 T was most closely related to Sphingomonas mali IFO 15500 T (95.1 %), Sphingomonas aquatilis JSS7 T (95.0 %), Sphingomonas pruni IFO 15498 T (94.9 %), Sphingomonas melonis DSM 14444 T (94.9 %) and Sphingomonas asaccharolytica IFO 15499 T (94.5 %). The major fatty acids of strain TDMA-16 T were C 17 : 1 ω 6 c (34.5 %) and C 18 : 1 ω 7 c (29.3 %). The presence of Q-10 as the main ubiquinone, the Sphingomonadaceae -specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus Sphingomonas sensu stricto . Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16 T from closely related Sphingomonas species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16 T represents a novel Sphingomonas species, for which the name Sphingomonas jaspsi sp. nov. is proposed. The type strain is strain TDMA-16 T (=NBRC 102120 T =DSM 18422 T =CCUG 53607 T ).