Determination of codeine, 6-methoxycodeine and thebaine using capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II) chemiluminescence detection
1998; Royal Society of Chemistry; Volume: 35; Issue: 10 Linguagem: Inglês
10.1039/a805955a
ISSN1364-5536
AutoresNeil W. Barnett, Benjamin J. Hindson, Simon W. Lewis, Stuart D. Purcell,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoA simple and robust capillary electrophoresis chemiluminescence detection system for the determination of codeine, 6-methoxycodeine and thebaine is described, based upon the reaction of these analytes with chemically generated tris(2,2′-bipyridyl)ruthenium(III) prepared in sulfuric acid (0.05 M). The reagent solution was contained in a glass detection cell which also held both the capillary and the cathode. The resultant chemiluminescence was monitored directly using a photomultiplier tube mounted flush against the base of the detection cell. The methodology, which incorporated a field amplification sample introduction procedure, realised detection limits (3σ baseline noise) of 5 × 10–8M for both codeine and 6-methoxycodeine and 1 × 10–7M for thebaine. The relative standard deviations of the migration times and the peak areas for the three analytes ranged from 2.2 up to 2.5% and 1.9 up to 4.6%, respectively.
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