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Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism

2003; American Society for Microbiology; Volume: 41; Issue: 1 Linguagem: Inglês

10.1128/jcm.41.1.506-508.2003

1098-660X

Duncan M. Kuhn, Maher M. Balkis, J. Chandra, Pranab K. Mukherjee, Mahmoud A. Ghannoum,

Microbial Natural Products and Biosynthesis

ABSTRACT Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT {2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide} and WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium] and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.