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A Single-Nucleotide Synonymous Mutation in the gag Gene Controlling Human Immunodeficiency Virus Type 1 Virion Production

2006; American Society for Microbiology; Volume: 81; Issue: 3 Linguagem: Inglês

10.1128/jvi.01596-06

1098-5514

Takaichi Hamano, Kazuhiro Matsuo, Yurina Hibi, Ann Florence B. Victoriano, Naoko Takahashi, Yosio Mabuchi, Tśuyoshi Soji, Shinji Irie, Pathom Sawanpanyalert, Hideki Yanai, Takashi Hara, Shudo Yamazaki, Naoki Yamamoto, Takashi Okamoto,

HIV/AIDS Research and Interventions

Viral factors as well as host ones play major roles in the disease progression of human immunodeficiency virus type 1 (HIV-1) infection. We have examined cytotoxic T-lymphocyte activity and HIV-1 DNA PCR results of 312 high-risk seronegative drug users in northern Thailand and identified four seronegative cases positive for both assays. Furthermore, we have identified a synonymous mutation in nucleotide position 75 of the gag p17 gene (A426G) of HIV-1 that belongs to the CRF01_AE virus circulating in Thailand. The replication-competent HIV-1 clone containing the A426G mutation demonstrated a dramatic reduction of virion production and perturbation of viral morphogenesis without affecting viral protein synthesis in cells.