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GMab-1, a high-affinity anti-3′-isoLM1/3′,6′-isoLD1 IgG monoclonal antibody, raised in lacto-series ganglioside-defective knockout mice

2009; Elsevier BV; Volume: 391; Issue: 1 Linguagem: Inglês

10.1016/j.bbrc.2009.11.132

1090-2104

Yukinari Kato, Chien-Tsun Kuan, Jinli Chang, Mika K. Kaneko, Joanne Ayriss, Hailan Piao, Vidyalakshmi Chandramohan, Charles N. Pegram, Roger E. McLendon, Pam Fredman, Jan‐Eric Månsson, Darell D. Bigner,

Toxin Mechanisms and Immunotoxins

The lacto-series gangliosides 3′-isoLM1 and 3′,6′-isoLD1 have been identified as tumor-associated antigens whose formation is initiated by the Lc3-synthase. Until now, high-affinity IgG monoclonal antibodies (mAbs) against 3′-isoLM1 and 3′,6′-isoLD1, which are highly expressed in gliomas, have not been developed, although mAbs against lacto-series gangliosides are powerful tools for functional studies. We previously produced the Lc3-synthase gene β3Gn-T5 knockout mice. In this study, we immunized β3Gn-T5 knockout mice with 3′-isoLM1/3′,6′-isoLD1 and produced the anti-3′-isoLM1/3′,6′-isoLD1 mAb GMab-1, of the IgG3 subclass, which should be useful for functional analysis of lacto-series gangliosides and for antibody-based therapy of gliomas.