Produção Nacional
Revisado por pares
Antioxidant Effects of Quercetin and Catechin Encapsulated into PLGA Nanoparticles
2012; Hindawi Publishing Corporation; Volume: 2012; Issue: 1 Linguagem: Inglês
10.1155/2012/145380
ISSN1687-4129
AutoresHéctor Pool, David Quintanar, Juan de Dios Figueroa, Camila Marinho Mano, Etelvino José Henriques Bechara, Luis A. Godı́nez, Sandra Mendoza,
Tópico(s)Seaweed-derived Bioactive Compounds
ResumoPolymeric nanoparticles (PLGA) have been developed for the encapsulation and controlled release of quercetin and catechin. Nanoparticles were fabricated using a solvent displacement method. Physicochemical properties were measured by light scattering, scanning electron microscopy and ζ ‐potential, X‐ray diffraction, infrared spectroscopy and differential scanning calorimetry. Encapsulation efficiency and in vitro release profiles were obtained from differential pulse voltammetry experiments. Antioxidant properties of free and encapsulated flavonoids were determined by TBARS, fluorescence spectroscopy and standard chelating activity methods. Relatively small ( d≈ 400 nm) polymeric nanoparticles were obtained containing quercetin or catechin in a non‐crystalline form (EE ≈ 79%) and the main interactions between the polymer and each flavonoid were found to consist of hydrogen bonds. In vitro release profiles were pH‐dependant, the more acidic pH, the faster release of each flavonoid from the polymeric nanoparticles. The inhibition of the action of free radicals and chelating properties, were also enhanced when quercetin and catechin were encapsulated within PLGA nanoparticles. The information obtained from this study will facilitate the design and fabrication of polymeric nanoparticles as possible oral delivery systems for encapsulation, protection and controlled release of flavonoids aimed to prevent oxidative stress in human body or food products.
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