Role of breast regression protein 39 (BRP-39)/chitinase 3-like-1 in Th2 and IL-13–induced tissue responses and apoptosis
2009; Rockefeller University Press; Volume: 206; Issue: 5 Linguagem: Inglês
10.1084/jem.20081271
ISSN1540-9538
AutoresChun Geun Lee, Dominik Hartl, Gap Ryol Lee, Barbara Koller, Hiroshi Matsuura, Carla A. Da Silva, Myung Hyun Sohn, Lauren Cohn, Robert Homer, Alexander Kozhich, Alison A. Humbles, Jennifer Kearley, Anthony J. Coyle, Geoffrey Chupp, Jennifer L. Reed, Richard A. Flavell, Jack A. Elias,
Tópico(s)Nanocomposite Films for Food Packaging
ResumoMouse breast regression protein 39 (BRP-39; Chi3l1) and its human homologue YKL-40 are chitinase-like proteins that lack chitinase activity. Although YKL-40 is expressed in exaggerated quantities and correlates with disease activity in asthma and many other disorders, the biological properties of BRP-39/YKL-40 have only been rudimentarily defined. We describe the generation and characterization of BRP-39−/− mice, YKL-40 transgenic mice, and mice that lack BRP-39 and produce YKL-40 only in their pulmonary epithelium. Studies of these mice demonstrated that BRP-39−/− animals have markedly diminished antigen-induced Th2 responses and that epithelial YKL-40 rescues the Th2 responses in these animals. The ability of interleukin13 to induce tissue inflammation and fibrosis was also markedly diminished in the absence of BRP-39. Mechanistic investigations demonstrated that BRP-39 and YKL-40 play an essential role in antigen sensitization and immunoglobulin E induction, stimulate dendritic cell accumulation and activation, and induce alternative macrophage activation. These proteins also inhibit inflammatory cell apoptosis/cell death while inhibiting Fas expression, activating protein kinase B/AKT, and inducing Faim 3. These studies establish novel regulatory roles for BRP-39/YKL-40 in the initiation and effector phases of Th2 inflammation and remodeling and suggest that these proteins are therapeutic targets in Th2- and macrophage-mediated disorders.
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