Produção Nacional
Revisado por pares
Experimental model of human corpus cavernosum smooth muscle relaxation
2010; Sociedade Brasileira de Urologia; Volume: 36; Issue: 4 Linguagem: Inglês
10.1590/s1677-55382010000400012
ISSN1677-6119
AutoresRommel Prata Regadas, Maria Elisabete Amaral de Moraes, Francisco José Cabral Mesquita, João Batista Gadelha de Cerqueira, Lúcio Flávio Gonzaga-Silva,
Tópico(s)Urinary Bladder and Prostate Research
ResumoTo describe a technique for en bloc harvesting of the corpus cavernosum, cavernous artery and urethra from transplant organ donors and contraction-relaxation experiments with corpus cavernosum smooth muscle.The corpus cavernosum was dissected to the point of attachment with the crus penis. A 3 cm segment (corpus cavernosum and urethra) was isolated and placed in ice-cold sterile transportation buffer. Under magnification, the cavernous artery was dissected. Thus, 2 cm fragments of cavernous artery and corpus cavernosum were obtained. Strips measuring 3 x 3 x 8 mm(3) were then mounted vertically in an isolated organ bath device. Contractions were measured isometrically with a Narco-Biosystems force displacement transducer (model F-60, Narco-Biosystems, Houston, TX, USA) and recorded on a 4-channel Narco-Biosystems desk model polygraph.Phenylephrine (1 microM) was used to induce tonic contractions in the corpus cavernosum (3-5 g tension) and cavernous artery (0.5-1 g tension) until reaching a plateau. After precontraction, smooth muscle relaxants were used to produce relaxation-response curves (10(-12) M to 10(-4) M). Sodium nitroprusside was used as a relaxation control.The harvesting technique and the smooth muscle contraction-relaxation model described in this study were shown to be useful instruments in the search for new drugs for the treatment of human erectile dysfunction.
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