The STAT3-Binding Long Noncoding RNA lnc-DC Controls Human Dendritic Cell Differentiation
2014; American Association for the Advancement of Science; Volume: 344; Issue: 6181 Linguagem: Inglês
10.1126/science.1251456
ISSN1095-9203
AutoresPin Wang, Yiquan Xue, Yanmei Han, Lin Li, Cong Wu, Sheng Xu, Zhengping Jiang, Junfang Xu, Qiuyan Liu, Xuetao Cao,
Tópico(s)interferon and immune responses
ResumoLong noncoding RNAs (lncRNAs) play important roles in diverse biological processes; however, few have been identified that regulate immune cell differentiation and function. Here, we identified lnc-DC, which was exclusively expressed in human conventional dendritic cells (DCs). Knockdown of lnc-DC impaired DC differentiation from human monocytes in vitro and from mouse bone marrow cells in vivo and reduced capacity of DCs to stimulate T cell activation. lnc-DC mediated these effects by activating the transcription factor STAT3 (signal transducer and activator of transcription 3). lnc-DC bound directly to STAT3 in the cytoplasm, which promoted STAT3 phosphorylation on tyrosine-705 by preventing STAT3 binding to and dephosphorylation by SHP1. Our work identifies a lncRNA that regulates DC differentiation and also broadens the known mechanisms of lncRNA action.
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