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Cryptosporidium Species and Genotypes in HIV‐Positive Patients in Lima, Peru

2003; Wiley; Volume: 50; Issue: s1 Linguagem: Inglês

10.1111/j.1550-7408.2003.tb00620.x

1550-7408

Vitaliano Cama, Caryn Bern, Irshad M. Sulaiman, Robert H. Gilman, Eduardo Ticona, Aldo Vivar, Vivian Kawai, Daniel Vargas, Ling Zhou, Lihua Xiao,

Amoebic Infections and Treatments

ABSTRACT: Cryptosporidium parasites from a cross‐sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to deteimine the diversity of Cryptosporidium spp. in HIV‐positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium ‐ positive specimens from 300 patients using a small subunit rRNA‐based PCR‐RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV‐positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.