Artigo Revisado por pares

Photochemically Initiated Protein Splicing

1995; Wiley; Volume: 34; Issue: 15 Linguagem: Inglês

10.1002/anie.199516291

ISSN

1521-3773

Autores

Sandra N. Cook, William E. Jack, Xiao‐Feng Xiong, Lora E. Danley, Jonathan A. Ellman, Peter G. Schultz, Christopher J. Noren,

Tópico(s)

Bacterial Genetics and Biotechnology

Resumo

More details on the mechanism of protein splicing were revealed from the substitution of O ‐(2‐nitrobenzyl)serine for the conserved Ser1082 at the upstream splice junction of the self‐splicing DNA polymerase of Thermococcus litoralis . Full length precursor protein was produced, which underwent protein splicing only upon photolysis.

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