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Effect of experimental diabetes on the development and maintenance of vulvovaginal candidiasis in female rats

2009; Elsevier BV; Volume: 200; Issue: 6 Linguagem: Inglês

10.1016/j.ajog.2009.01.011

1097-6868

Márcia Aparecida Carrara, Roberto Barbosa Bazotte, Lucélia Donatti, Terezinha Inez Estivalet Svidzinski, Márcia Edilaine Lopes Consolaro, Eliana Valéria Patussi, Márcia Regina Batista,

Diabetes and associated disorders

Objective The purpose of this study was to develop an experimental model of diabetes in female rats and verify its influence on vulvovaginal candidiasis. Study Design The animals were divided into control and diabetic groups. Diabetes was induced with the use of an intravenous solution of alloxan (42 mg/kg bodyweight). One week after confirmation of hyperglycemia, the inoculation of Candida albicans yeast, previously standardized from a vaginal isolate, in concentrations of about 5 × 108, was performed. Infection control was made through vaginal culture, Papanicolaou cytology, and scanning electron microscopy (SCEM). Results The results pointed to different glycemias between the control (74.8 ± 2.6) and experimental groups (543.1 ± 12.1) and a significant bodyweight decrease (227.6 ± 4.77 and 204 ± 6.39, respectively). The positive infection was shown by culture, Papanicolaou test, and SCEM in the experimental group. Conclusion Diabetes mellitus causes hyperglycemia, which was favorable to the vaginal colonization and infection by C albicans. The purpose of this study was to develop an experimental model of diabetes in female rats and verify its influence on vulvovaginal candidiasis. The animals were divided into control and diabetic groups. Diabetes was induced with the use of an intravenous solution of alloxan (42 mg/kg bodyweight). One week after confirmation of hyperglycemia, the inoculation of Candida albicans yeast, previously standardized from a vaginal isolate, in concentrations of about 5 × 108, was performed. Infection control was made through vaginal culture, Papanicolaou cytology, and scanning electron microscopy (SCEM). The results pointed to different glycemias between the control (74.8 ± 2.6) and experimental groups (543.1 ± 12.1) and a significant bodyweight decrease (227.6 ± 4.77 and 204 ± 6.39, respectively). The positive infection was shown by culture, Papanicolaou test, and SCEM in the experimental group. Diabetes mellitus causes hyperglycemia, which was favorable to the vaginal colonization and infection by C albicans.