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BIBTEX RIS

Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil

2014; Elsevier BV; Volume: 25; Linguagem: Inglês

10.1016/j.ijid.2014.01.005

1878-3511

Franciéli Pedrotti Rozales, Vanessa Bley Ribeiro, Cibele Massotti Magagnin, Mariana Pagano, Larissa Lutz, Diego R. Falci, Adão Rogério Leal Machado, Afonso Luís Barth, Alexandre Prehn Zavascki,

Pharmaceutical and Antibiotic Environmental Impacts

To evaluate the emergence of New Delhi metallo-β-lactamase 1 (NDM-1)-producing Enterobacteriaceae isolates in Brazil.From April to October 2013, following the detection of the first NDM-1-producing isolate, a surveillance study was performed for the detection of blaNDM-1 among Enterobacteriaceae isolates with reduced susceptibility to carbapenems in 17 hospitals of Porto Alegre, Brazil. Real-time PCR was used to determine the presence of carbapenemase genes, which were further sequenced. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE).A total of 1134 isolates were evaluated. blaNDM-1 was detected in 11 (0.97%) isolates: nine Enterobacter cloacae complex (eight belonging to a single clone recovered from two distinct hospitals and the other strain from a third hospital) and two Morganella morganii (belonging to a single clone recovered from one hospital). Most isolates presented high-level resistance to carbapenems.NDM-1-producing Enterobacteriaceae have emerged rapidly in the hospitals of the Brazilian city where they were first detected. The emergence of NDM-1 in Brazil is of great concern, since it is a severe threat to antimicrobial therapy against Enterobacteriaceae in this country.