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gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi

1994; American Society for Microbiology; Volume: 176; Issue: 10 Linguagem: Inglês

10.1128/jb.176.10.3072-3075.1994

1098-5530

D. Scott Samuels, Richard T. Marconi, W M Huang, Claude F. Garon,

Insect Resistance and Genetics

We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.