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Increase of total protein synthesis during mouse oocyte growth

1979; Bioscientifica; Volume: 57; Issue: 2 Linguagem: Inglês

10.1530/jrf.0.0570405

1741-7899

Rita Canipari, A. Pietrolucci, F. Mangia,

Mass Spectrometry Techniques and Applications

Summary. Quantitative changes of mouse oocyte protein synthesis during oogenesis have been studied by determining the rate of leucine incorporation into total cell protein. The leucine intracellular pool was artificially expanded by exposing the isolated oocytes to different concentrations of radiolabelled leucine, and leucine incorporation was followed as a function of the time. Protein synthesis by mouse oocytes increases linearly with the increase of the cell volume, and such increase is constant throughout the entire period of oocyte growth.