Cutting Edge: Suppression of GM-CSF Expression in Murine and Human T Cells by IL-27
2012; American Association of Immunologists; Volume: 189; Issue: 5 Linguagem: Inglês
10.4049/jimmunol.1200131
ISSN1550-6606
AutoresA. B. Young, Eimear Linehan, Emily Hams, Aisling O’Hara Hall, Angela McClurg, James A. Johnston, Christopher A. Hunter, Padraic G. Fallon, Denise Fitzgerald,
Tópico(s)Immune Response and Inflammation
ResumoGM-CSF is a potent proinflammatory cytokine that plays a pathogenic role in the CNS inflammatory disease experimental autoimmune encephalomyelitis. As IL-27 alleviates experimental autoimmune encephalomyelitis, we hypothesized that IL-27 suppresses GM-CSF expression by T cells. We found that IL-27 suppressed GM-CSF expression in CD4+ and CD8+ T cells in splenocyte and purified T cell cultures. IL-27 suppressed GM-CSF in Th1, but not Th17, cells. IL-27 also suppressed GM-CSF expression by human T cells in nonpolarized and Th1- but not Th17-polarized PBMC cultures. In vivo, IL-27p28 deficiency resulted in increased GM-CSF expression by CNS-infiltrating T cells during Toxoplasma gondii infection. Although in vitro suppression of GM-CSF by IL-27 was independent of IL-2 suppression, IL-10 upregulation, or SOCS3 signaling, we observed that IL-27-driven suppression of GM-CSF was STAT1 dependent. Our findings demonstrate that IL-27 is a robust negative regulator of GM-CSF expression in T cells, which likely inhibits T cell pathogenicity in CNS inflammation.
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