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Significant Improvement of the Recombinant Borrelia -Specific Immunoglobulin G Immunoblot Test by Addition of VlsE and a DbpA Homologue Derived from Borrelia garinii for Diagnosis of Early Neuroborreliosis

2003; American Society for Microbiology; Volume: 41; Issue: 3 Linguagem: Inglês

10.1128/jcm.41.3.1299-1303.2003

1098-660X

Ulrike Schulte-Spechtel, Gisela Lehnert, Gaby Liegl, Volker Fingerle, Christiane Heimerl, Barbara J. B. Johnson, Bettina Wilske,

Vector-Borne Animal Diseases

ABSTRACT We investigated whether the recombinant Borrelia Western blot test previously described (B. Wilske, C. Habermann, V. Fingerle, B. Hillenbrand, S. Jauris-Heipke, G. Lehnert, I. Pradel, D. Rössler, and U. Schulte-Spechtel, Med. Microbiol. Immunol. 188: 139-144, 1999) can be improved by the addition of VlsE and additional DbpA and OspC homologues. By using a panel of sera from 36 neuroborreliosis patients and 67 control patients, the diagnostic sensitivity of the recombinant immunoblot test was significantly increased (86.1% versus 52.7%) without loss of specificity and was higher (86.1% versus 63.8%) than that of the conventional whole-cell lysate immunoblot test (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35: 1433-1444, 1997). Improvement was mainly due to the presence of VlsE and DbpA.