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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis

2009; Wiley; Volume: 40; Issue: 3 Linguagem: Inglês

10.1002/eji.200939747

ISSN

1521-4141

Autores

Lucas de Lima Nogueira, Fernanda C. Cardoso, Ana Márcia Menezes de Mattos, Juliano Bordignon, Cláudia P. Figueiredo, Pedro Dahlstrom, Cristiane Cunha Frota, Claudia Nunes Duarte dos Santos, Marcelo Chalhoub, Benildo Sousa Cavada, Henrique Couto Teixeira, Sérgio C. Oliveira, Manoel Barral‐Netto, André Báfica,

Tópico(s)

Glycosylation and Glycoproteins Research

Resumo

In this study, we have identified a secreted 13 kDa lectin from Mtb (Mtb, Mycobacterium tuberculosis; sMTL-13) by homology search of a non-redundant lectin database. Bioinformatic analysis revealed that sMTL-13 belongs to the ricin-type beta-trefoil family of proteins containing a Sec-type signal peptide present in Mtb complex species, but not in non-tuberculous mycobacteria. Following heterologous expression of sMTL-13 and generation of an mAb (clone 276.B7/IgG1kappa), we confirmed that this lectin is present in culture filtrate proteins from Mtb H37Rv, but not in non-tuberculous mycobacteria-derived culture filtrate proteins. In addition, sMTL-13 leads to an increased IFN-gamma production by PBMC from active tuberculosis (ATB) patients. Furthermore, sera from ATB patients displayed high titers of IgG Ab against sMTL-13, a response found to be decreased following successful anti-tuberculosis therapy. Together, our findings reveal a secreted 13 kDa ricin-like lectin from Mtb, which is immunologically recognized during ATB and could serve as a biomarker of disease treatment.

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