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Yellow fever virus envelope protein has two discrete type-specific neutralizing epitopes.

1997; Microbiology Society; Volume: 78; Issue: 6 Linguagem: Inglês

10.1099/0022-1317-78-6-1353

1465-2099

Kate D. Ryman, Alan D.T. Barrett, J. J. Schlesinger, Ronald C. Weir, T.N. Ledger,

Virology and Viral Diseases

Two monoclonal antibody neutralization resistant (MAbR) variants of the yellow fever (YF) 17D-204 vaccine virus strain were selected using YF type-specific MAb B39. These B39R variants were compared with the variant virus selected by Lobigs et al. (Virology 161, 474-478, 1987) using a second YF-type specific MAb (2E10) which mapped to amino acid position 71/72 in the envelope (E) protein. Neutralization assays with a panel of MAbs suggested that these two YF-type-specific epitopes are located in two discrete regions of the folded E protein. Each of the B39R variants had a single nucleotide mutation which encoded an amino acid substitution at either position E-155 or E-158. Thus, YF type-specific epitopes map to both domain I (B39) and II (2E10) of the YF virus E protein. The B39 defined epitope represents the first flavivirus neutralizing epitope localized to this region of domain I of the E protein.