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Recovery of Drugs of Abuse from the Immunalysis Quantisal Oral Fluid Collection Device

2006; Oxford University Press; Volume: 30; Issue: 8 Linguagem: Inglês

10.1093/jat/30.8.614

1945-2403

O. Quintela, Dennis J. Crouch, David M. Andrenyak,

Salivary Gland Disorders and Functions

Drug recovery from a new oral fluid collection device was assessed. The evaluation was performed in vitro at three physiologically relevant concentrations for the following substances: amphetamine, methamphetamine, morphine, codeine, cocaine, benzoylecgonine, methadone, oxazepam, and Δ9-tetrahydrocannabinol (THC). Drug-free and drug-fortified controls were prepared and their concentration verified by liquid chromatography-tandem mass spectrometry. Aliquots of the controls were then "collected" with the device (n = 3) using the manufacturer's recommended procedure. Collected samples were stored for 12 h to simulate shipping before analysis. Fresh, non-"collected" aliquots of each pool (n = 3) were concurrently analyzed. The drug recoveries from the Quantisal were expressed as a mean percentage of the concurrently analyzed aliquots that were not subjected to device collection. Recoveries for oxazepam exceeded 97%, for amphetamine and methamphetamine exceeded 93%, and for opioids all exceeded 91%. The recoveries of cocaine were > 91% and > 82% for its polar metabolite, benzoylecgonine. Especially noteworthy was the recovery of THC from the Quantisal collector (81.3–91.4%). When compared with recoveries reported from other collection devices, the Quantisal was clearly superior.