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Improvement of fertilization and pregnancy rate after intracytoplasmic fine morphologically selected sperm injection

2004; Elsevier BV; Volume: 82; Linguagem: Inglês

10.1016/j.fertnstert.2004.07.449

1556-5653

Anne Marie Junca, P. Cohen-Bacrie, A. Hazout,

Assisted Reproductive Technology and Twin Pregnancy

ObjectiveIntracytoplasmic sperm injection ( ICSI ) is the most efficient technique in case of male infertility.A new method of unstained , real-time high magnification ( × 6600 ) motile sperm organellar morphology examination ( MSOME ) was developed by BARTOOV and al (J.Androl.2002 ;23 ). Application of this new method to patients undergoing ICSI demonstrated that fine morphological integrity of the human sperm nuclei is an important parameter associated with ICSI pregnancy rate .DesignComparative prospective study testing routine ICSI outcome parameters against those of modified ICSI based on morphological selection of spermatozoa with normal nuclei.Materials and methodsIMSI (Intracytoplasmic Morphologycally Selected sperm Injection), was applied to patients with at least 2 previous unsuccess attempts ICSI (27 cases) or with severe teratospermia (<14%) (17 cases). All partners were younger than 38 years. The technical procedure of IMSI was conducted according to the method already described by Bartoov et al. Briefly, motile spermatozoa was selected by two layers density gradient and the supernatant was suspended in culture medium. An aliquot of 2μl of the sperm cell suspension was transferred into 5 μl micro droplet ofSpermCatch® (Hyaluronic acid + Human Serum Albumin ) under mineral oil in a sterile glass bottom dish and placed on microscopic stage over the top of an objective lens previously covered by a droplet of immersion oil. Sperm selection was realized at high magnification ( × 6600 ) by the inverted microscope ( Olympus IX 70 ) equiped with Nomarski differential interference contrast optics. RESULTS:* terminated in a first trimester missed abortion.Conclusion ObjectiveIntracytoplasmic sperm injection ( ICSI ) is the most efficient technique in case of male infertility.A new method of unstained , real-time high magnification ( × 6600 ) motile sperm organellar morphology examination ( MSOME ) was developed by BARTOOV and al (J.Androl.2002 ;23 ). Application of this new method to patients undergoing ICSI demonstrated that fine morphological integrity of the human sperm nuclei is an important parameter associated with ICSI pregnancy rate . Intracytoplasmic sperm injection ( ICSI ) is the most efficient technique in case of male infertility.A new method of unstained , real-time high magnification ( × 6600 ) motile sperm organellar morphology examination ( MSOME ) was developed by BARTOOV and al (J.Androl.2002 ;23 ). Application of this new method to patients undergoing ICSI demonstrated that fine morphological integrity of the human sperm nuclei is an important parameter associated with ICSI pregnancy rate . DesignComparative prospective study testing routine ICSI outcome parameters against those of modified ICSI based on morphological selection of spermatozoa with normal nuclei. Comparative prospective study testing routine ICSI outcome parameters against those of modified ICSI based on morphological selection of spermatozoa with normal nuclei. Materials and methodsIMSI (Intracytoplasmic Morphologycally Selected sperm Injection), was applied to patients with at least 2 previous unsuccess attempts ICSI (27 cases) or with severe teratospermia (<14%) (17 cases). All partners were younger than 38 years. The technical procedure of IMSI was conducted according to the method already described by Bartoov et al. Briefly, motile spermatozoa was selected by two layers density gradient and the supernatant was suspended in culture medium. An aliquot of 2μl of the sperm cell suspension was transferred into 5 μl micro droplet ofSpermCatch® (Hyaluronic acid + Human Serum Albumin ) under mineral oil in a sterile glass bottom dish and placed on microscopic stage over the top of an objective lens previously covered by a droplet of immersion oil. Sperm selection was realized at high magnification ( × 6600 ) by the inverted microscope ( Olympus IX 70 ) equiped with Nomarski differential interference contrast optics. RESULTS:* terminated in a first trimester missed abortion. IMSI (Intracytoplasmic Morphologycally Selected sperm Injection), was applied to patients with at least 2 previous unsuccess attempts ICSI (27 cases) or with severe teratospermia (<14%) (17 cases). All partners were younger than 38 years. The technical procedure of IMSI was conducted according to the method already described by Bartoov et al. Briefly, motile spermatozoa was selected by two layers density gradient and the supernatant was suspended in culture medium. An aliquot of 2μl of the sperm cell suspension was transferred into 5 μl micro droplet ofSpermCatch® (Hyaluronic acid + Human Serum Albumin ) under mineral oil in a sterile glass bottom dish and placed on microscopic stage over the top of an objective lens previously covered by a droplet of immersion oil. Sperm selection was realized at high magnification ( × 6600 ) by the inverted microscope ( Olympus IX 70 ) equiped with Nomarski differential interference contrast optics. RESULTS:* terminated in a first trimester missed abortion. Conclusion