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Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia

2011; Elsevier BV; Volume: 92; Issue: 1 Linguagem: Inglês

10.1016/j.antiviral.2011.07.009

1872-9096

Francielle Tramontini Gomes de Sousa, Carla Maísa Camelini, Alessandra Mascarello, Márcio José Rossi, Ricardo José Nunes, Célia Regina Monte Barardi, Margarida Matos de Mendonça, Cláudia Maria Oliveira Simões,

Polysaccharides and Plant Cell Walls

Sulfated polysaccharides are good candidates for drug discovery in the treatment of herpetic infections. Agaricus brasiliensis (syn A. subrufescens, A. blazei) is a Basidiomycete fungus native to the Atlantic forest region of Southeastern Brazil. Herein we report the chemical modification of a polysaccharide extracted from A. brasiliensis mycelia to obtain its sulfated derivative (MI-S), which presented a promising inhibitory activity against HSV-1 [KOS and 29R (acyclovir-resistant) strains] and HSV-2 strain 333, with selectivity indices (SI = CC50/IC50) higher than 439, 208, and 562, respectively. The mechanisms underlying this inhibitory activity were scrutinized by plaque assay with different methodological strategies. MI-S had no virucidal effects, but inhibited HSV-1 and HSV-2 attachment, penetration, and cell-to-cell spread, as well as reducing the expression of HSV-1 ICP27, UL42, gB, and gD proteins. MI-S also presented synergistic antiviral effect with acyclovir. These results suggest that MI-S presents multiple modes of anti-HSV action.