Portal de Periódicos da CAPES

Assessment of Cytokines by Immunofluorescence and the Paraformaldehyde‐Saponin Procedure

1991; Wiley; Volume: 119; Issue: 1 Linguagem: Inglês

10.1111/j.1600-065x.1991.tb00578.x

1600-065X

Birgitta Sander, Jan Andersson, Jan Andersson,

Immune Cell Function and Interaction

Immunological ReviewsVolume 119, Issue 1 p. 65-93 Assessment of Cytokines by Immunofluorescence and the Paraformaldehyde-Saponin Procedure Birgitta Sander, Birgitta Sander Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this authorJan Andersson, Jan Andersson Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this authorUlf Andersson, Ulf Andersson Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this author Birgitta Sander, Birgitta Sander Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this authorJan Andersson, Jan Andersson Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this authorUlf Andersson, Ulf Andersson Dept. of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, S-106 91 Stockholm, Sweden.Search for more papers by this author First published: February 1991 https://doi.org/10.1111/j.1600-065X.1991.tb00578.xCitations: 343AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat REFERENCES Alfa, J. M., Dembinsky, J. J. & Jay, F. T. (1987) Production of monoclonal antibodies against recombinant human gamma interferon: a method for the screening of hybridomas without purified antigens. Hybridoma 6, 509. Andersson, G., Ekre, H-P., Aim, G. & Perlmann, P. (1989) Monoclonal antibody two-site ELISA for human IFN-gamma. Adaptation for determinants in human serum or plasma. J. Immunol. Methods 125, 89. Andersson, J. & Andersson, U. (1990) Human intra-venous immunoglobulin modulates motiokine production in vivo. Immunology (in press). Andersson, U. (1987) Regulation of antibody synthesis in the neonate. In; Immunology of the Neonate., eds. G. R. Burgio, L. A. Hanson & A. G. Ugazio, pp. 37–50. Springer Verlag, Heidelberg , Germany . Andersson, U., Adolf, G., Dohlsten, M., Molter, G. & Sjogren, H-O. (1989) Characterization of individual tumor necrosis factor alpha and beta producing cells after polyclonal T cell activation. J. Immunol. Methods 123, 233. Andersson, U., Andersson, J., Lindfors, A., Heusser, C. & Moller, G. (1991) Restricted cylokine production by human cord blood monuclear cells. In: Neonatal Hematology and Immunology, eds. R. Bracci & M. Xanthou, Elsevier Press, Amsterdam . Andersson, U., Andersson, J., Lindfors, A., Wagner, K., Moller, G. & Heusser, C. H. (1990) Simultaneous production of interleukin 2, interleukin 4 and interferon gamma by activated human blood lymphocytes. Eur. J. Immunol. 20, 1591. Andersson, U., Hallden, G., Persson, U., Hed, J., Möller, G. & DeLey, M. (1988) Enumeration of IEN-gamma-producing cells by flow cytometry: comparison with fluorescence microscopy. J. Immunol. Methods 112, 139. Andersson, U. & Matsuda, T. (1989) Human interleukin 6 and tumor necrosis factor alpha production studied at a single cell level. Eur. J. Immunol. 19, 1157. Andersson, U. & Sander, B. (1989) Detection of individual interleukin 2 producing cells after anti-CD3 antibody activation. Immunol letters 20, 115. Andersson, U., Sander, B., Andersson, J. & Moller, G. (1988) Concomitant production of different lymphokines in activated T cells. Eur. J. Immunol. 18, 2081. Bakouche, O., Brown, D. C. & Lachman, L. B. (1987) Subcellular localization of human monocyte interleukin 1: evidence for an inactive precursor molecule and a possible mechanism for IL 1 release. J. Immunol. 138, 4249. Bangham, A. D. & Home, R. W. (1962) Action of saponin on biological cell membranes. Nature 196, 952. Barkley, D., Andersson, U., Brennan, F. & Maini, R. N. (1990a) Profile of cytokine production by blood and synovial cells of patients with rheumatoid arthritis. (Submitted). Barkley, D., Feldmann, M. & Maini, R. N. (1989) The detection by immunofluorescence of distinct cell populations producing interIeukin-1 alpha and interleukin-1 beta in activated human peripheral blood. J. Immunol. Methods 120, 277. Barkley, D., Feldmann, M. & Maini, R. N. (1990b) Cells with dendritic morphology and interleukin-1 alpha staining circulate in the blood of patients with rheumatoid arthritis. Clin. Exp. Immunol. 80, 25. Berthold, W. & Zahn, G. (1984) Antiviral Res. ABSTR. 1: 69 (abstract). Bringman, T. S. & Aggarwal, B. B. (1987) Monoclonal antibodies to human tumor necrosis factor alpha and beta. Hybridoma 6, 489. Cardell, S. & Sander, B. (1990) Interleukin 2, 4 and 5 are sequenetelly produced in mitogen stimulated murine spleen cultures. Eur. J. Immunol. 20, 389. Chang, T. W., McKinney, S., Liu, V, Kung, P. C, Vilcek, J. & Le, J. (1984) Use of monoclonal antibodies as sensitive and specific probes for biologically active human gamma-interferon. Proc. Natl. Acad. Sci. USA 81, 5219. Conlon, P. J., Grabstein, K. H., Alpert, A., Prickett, K. S., Hopp, T. P. & Gillis, S. (1987) Localization of human mononuclear cell interleukin I. J. Immunol. 139, 98. Dialynas, D. P., Wilde, D. B., Marrack, P., Pierres, A., Wall, K. A., Havran, W., Otten, G., Loken, M. R., Pierres, M., Kappler, J. & Fitch, F. W. (1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GKI.5: Expression of L3T4a by functional T cell clones appear to correlate primary with class II MHC antigen reactivity. Immunol. Rev. 74, 29. Dourmashkin, R. R., Dougherty, R. M. & Harris, R. J. C. (1962) Electron microscopic observations on Rous sarcoma virus and cell membranes. Nature 194, 1116. Eberhard, B. A., Silverman, E. D., Laxer, R. M., Rose, V., Shore, A. & Andersson, U. (1990) Single cell measurement of cytokine production in Kawasaki disease. American College of Rheumatology (meeting abstract). Espevik, T. & Nissen-Meyer, J. (1986) A highly sensitive cell line WEHI 164 clone 13 for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95, 99. Espevik, T. & Nissen-Meyer, J. (1987) Tumor necrosis factor-like activity on paraformaldehyde-fixed monocyte monolayers. Immunology 61, 443. Firestein, G., Alvaro-Garcia, J. M. & Maki, R. (1990) Quantitative analysis of cytokine gene expression in rheumatoid arthritis. J. Immunol. 144, 3347. Fischer H., Dohlsten, M., Andersson, U., Hedlund, G., Ericsson, P-C, Hansson, J. & Sjogren, H. (1990) Production of TNF-alpha and TNF-beta by Staphyioccal enterotoxin A activated human T cells. J. Immunol. 144, 4663. Glauert, A. M., Dingle, J. T. & Lucy, J. A. (1962) Action of saponin on biological cell membranes. Nature 196, 953. Halldén, G., Andersson, U., Hed, J. & Johansson, S. G. O. (1989) A new membrane permeabilization method for the detection of intracellular antigens by flow cytometry. J. Immunol. Methods 124, 103. Harada, N., Takahashi, T, Matsumoto, M., Kinashi, T, Ohara, J., Kikuchi, Y, Koyama, N., Severinson, E., Yaoita, Y. Honjo, T. Yamaguchi, N., Tominaga, A. & Takatsu, K. (1987) Production of a monoclonal antibody useful in the molecular characterization of murine T-cell-replacing factor/B-cell growth factor II. Proc. Natl. Acad. Sci. (USA) 84, 4581. Hasler, F., Bluestein, H. G., ZvaiHer, N. J. & Epstein, L. B. (1984) Analysis of the defects responsible for the impaired regulation of Epstein-Barr virus-induced B cell proliferation by rheumatoid arthritis lymphocytes. J. Exp. Med. 157, 173. Hayat, M. A. (1981) Fixation for Electron Microscopy. Academic Press. New York . Henter, J-L, Soder, O. & Andersson, U. (1988) Identification of individual tumor necrosis factor/cachectin producing cells after lipopolysaccharide induction. Eur J. Immunol. 18, 983. Heremans, H., Dijkmans, R., Sobis, H., Vanderckhove, F. & Billiau, A. (1987) Regulation by interferons on the local inflammatory response to bacterial lipopolysaccharide. J. Immunol. 132, 1896. Hirano, T., Matsuda, T, Hosoi, K., Okano, A., Matsui, H. & Kishimoto, T. (1988) Absence of antiviral activity in recombinant B cell stimulatory factor 2 (BSF 2). Immunol. Letters 17, 41. Hofsli, E., Bakke, O., Nonstad, U. & Espevik, T. (1989) A flow cytometric and immunofluorescence microscopic study of tumor necrosis factor production and localization in human monocytes. Cell. Immunol. 122, 405. Ida, N., Sakurai, S., Hosaka, T, Hosoi, K., Kunimoto, X, Shimazu, T, Maniyama, T, Matsuura, Y. & Kohase, M. (1989) Establishment of strongly neutralizing monoclonal antibody to human interleukin-6 and its epitope analysis. Biochem. Biophys. Res. Com. 165, 728. Johnson, G. D., Davidson, R. S., McNamee, K. C, Russell, G., Goodwin, D. & Holborow, E. J. (1982) Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy. J. Immunol. Methods 55, 231. Kabilan, L., Andersson, G., Lolli, F., Ekre, H-R, Olsson, T. & Troye-Blomberg, M. (1990) Detection of intracellular expression and secretion of interferon-gamma at the single-cell level after activation of human T cells with tetanus toxoid in vitro. Eur, J. Immunol. 20, 1085. Karasuyama, H. & Melchers, F. (1988) Establishment of mouse cell lines which constitutively secrete large quantities of interleukin 2, 3, 4 or 5, using modified cDNA expression vectors. Eur, J. Immunol. 18, 97. Kasahara, T., Mukaida, N., Shinomiya, H., Imai, M., Matsushima, K., Wakasugi, H. & Nakano, K. (1987) Preparation and characterization of poiyclonal and monoclonal antibodies against human IL-1 alpha. J. Immunol. 138, 1804. Kenney, J. S., Masada, M. P., Eugui, E. M., Delusho, B. M., Mulkins, M. A. & Allison, A. C. (1987) Monoclonal antibodies to recombinant interleukin I bela. J. Immunol. 138, 4236. Kuhweide, R., Van Damme, J. & Ceuppens, J. L. (1990) Tumor necrosis factor-a and interleukin 6 synergistically induce T cell growth. Eur, J. Immunol. 20, 1019. Lamche, H. R. & Adolf, G. R, (1990) Highly sensitive enzyme immunoassays for antibodies to human tumor necrosis factor (TNFα) and lymphotoxin (TNFβ). J. Immunol. Methods 131, 283. Ledbetter, J. A. & Herzenberg, L. A. (1979) Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol. Rev. 47, 63. Leduc, E. H., Scott, G. B. & Avrameas, S. (1969) Ultrastructural localization of intracellular immune globulins in plasma cells and lymphoblasts by enzyme-labelled antibodies. J. Histochem. Cytochem. 12, 211. Lee, C. L. Y., Lee, S. H. S., Jay, F. T & Rozee, K. R. (1990) Immunobiological study of interferon-gamma-producing cells after staphylococcal enterotoxin B stimulation. Immunology 70, 94. Lewis, D. B., Prickett, K. S., Larsen, A., Grabstein, K., Weaver, M. & Wilson, C. (1988) Restricted production of interleukin 4 by activated human T cells. Proc. Natl. Acad. Sei. (USA) 85, 9743. Lotz, M., Tsoukas, C. D., Fong, S., Dinerello, C. A., Carson, D. A. & Vaughan, J. H. (1986) Release of lymphokines after Epstein Barr virus infection in vitro. J. Immunol. 136, 3636. Lucy, J. A. & Glauert, A. M. (1964) Structure and assembly of macromolecular lipid complexes composed of globular micelles. J. Mol. Biol. 8, 727. Lundgren, M., Persson, U., Larsson, P., Magnusson, C, Smith, C. I. E., Hammarstrom, L. & Severinsson, E. (1989) Interleukin 4 induces synthesis of IgE and IgG4 in human B cells. Eur. J. Immunol. 19, 1311. Matsuda, T., Hirano, T. & Kishimoto, T. (1988) Establishment of an interleukin 6 (IL-6)/B cell stimulator factor 2-dependent cell line and preparation of anti-lL-6 monoclonal antibodies. Eur J. Immunol. 18, 951. McLean, I. W. & Nakane, P. K. (1974) Peri odately sine-para formaldehyde fixative. A new fixative for immunoelectron microscopy. J. Histochem. Cytochem. 22, 1077. Morré, D. J. & Outracht, L. (1977) Dynamics of the Golgi apparatus: Membrane differentiation and membrane flow. Int. Rev. Cytol. 5, 61. Ohara, J. & Paul, W. E. (1985) Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature 315, 333. Ohtsuki, I., Manzi, R. M., Palade, G. E. & Jamieson, J. D. (1978) Entry of macromolecular tracers into cells fixed with low concentrations of aldehydes. Biol. Cell. 31, 119. Poo, W-J., Conrad, L. & Janeway, C. A. Jr. (1988) Receptor-directed focusing of lymphokine release by helper T cells. Nature 332, 378. Rubartelli, A., Cozzolino, F, Talio, M. & Sitia, R. (1990) A novel secretory pathway for interleukin I beta, a protein lacking a signal sequence. EM BO J. 9, 1503. Sander, B., Cardell, S., Heremans, H., Andersson, U. & Moiler, G. (1989) Detection of individual interleukin 4- and gamma interferon-producing murine spleen ceils after activation with T-cell mitogens. Scand. J. Immunol. 30, 315. Sander, B., Cardell, S. & Moller, E. (1990) Interieukin4and interferon gamma production in restimulated CD4+ and CDS+ ceils indicates memory type responsiveness. (Submitted). Sandvig, S., Laskay, T., Andersson, J., DeLey, M. & Andersson, U, (1987) Gamma- interferon is produced by CD3+ and CD3− lymphocytes, Immunol. Rev. 97, 51. Schumacher, J. H., O'Garra, A., Shrader, B., van Kimmcnadc, A., Bond, M. W., Mosmann, T. R. & Coffman, R. L. (1988) The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J. Immunol. 141, 1576. Seeman, P. (1974) Ullrastructure of membrane lesions in immune lysis, osmotic lysis and drug-induced lysis. Fed. Proc. 33, 2116. Singer, I. I., Scott, S., Haii, G., Limjuco, G., Chin, J. & Schmidt, J. A. (1988) Interleukin t beta is localized in the cytoplasmatic ground substance but is largely absent from the Golgi apparatus and plasma membranes of stimulated human monocytes., J. Exp. Med 167, 389. Stefanos, S., Wietzezkin, J., Catinot, L., Devos, R. & Jalkoff, R. (1985) Characterization of antibodies against recombinant hIFN gamma produced by hybridoma cells. J. Interferon Res. 5, 455. Tartakoff, A. M. (1983) Perturbation of vesicular traffic with the carboxylic ionophore Monensin. Cell 32, 1026. Turner, M. & Feldmann, M. (1988) Comparison of patterns of expression of tumor necrosis factor, lymphotoxin and interleukin-6 mRNA. Biochem. Biophys. Res. Com. 153, 1144. Virtanen, L, Ekbtom, P. & Lauria, P. (1980) Subcellular compartmentalization of saecharide moieties in cultured normal and malignant cells. J. Cell. Biol. 85, 429. Willingham, M. C. (1980) Eleetron mieroswcopic immunocytochemical localization of intracellular antigens in altured ceils: the EGS and ferritin bridge procedures. Histo- chem. J. 12, 419. Willingham, M. C. (1983) An alternative fixation-processing method for preembedding ultrastructural immunocytochemistry of cytoplasmic antigens: the GBS (glutaraldehyde-borohydride-saponin) procedure. J. Histochem. Cytochem. 31, 791. M. C. Willingham & I. Pastan, (Eds.) (1985) An atlas of immunofluorescence in cultured cells, vol. II. Academic Press, Orlando . Citing Literature Volume119, Issue1February 1991Pages 65-93 ReferencesRelatedInformation