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Microtubule bending and breaking in living fibroblast cells

1999; The Company of Biologists; Volume: 112; Issue: 19 Linguagem: Inglês

10.1242/jcs.112.19.3283

1477-9137

David J. Odde, Le Ma, Amelie H. Briggs, Alyssa L. DeMarco, Marc W. Kirschner,

Skin and Cellular Biology Research

ABSTRACT Microtubules in living cells frequently bend and occasionally break, suggesting that relatively strong forces act on them. Bending implies an increase in microtubule lattice energy, which could in turn affect the kinetics and thermodynamics of microtubule-associated processes such as breaking. Here we show that the rate of microtubule breaking in fibroblast cells increases ∼40-fold as the elastic energy stored in curved microtubules increases to >∼1 kT/tubulin dimer. In addition, the length-normalized breaking rate is sufficiently large (2.3 breaksmm−1minute−1) to infer that breaking is likely a major mechanism by which noncentrosomal microtubules are generated. Together the results suggest a physiologically important, microtubule-based mechanism for mechanochemical information processing in the cell.