IA1 is NGN3-dependent and essential for differentiation of the endocrine pancreas
2006; Springer Nature; Volume: 25; Issue: 6 Linguagem: Inglês
10.1038/sj.emboj.7601011
ISSN1460-2075
AutoresGeorg Mellitzer, Stefan Bonné, Reini F. Luco, Mark Van de Casteele, Nathalie Lenne‐Samuel, Patrick Collombat, Ahmed Mansouri, Jacqueline Lee, Michael S. Lan, Daniël Pipeleers, Finn Cilius Nielsen, Jorge Ferrer, Gérard Gradwohl, Harry Heimberg,
Tópico(s)Genetics and Neurodevelopmental Disorders
ResumoArticle2 March 2006free access IA1 is NGN3-dependent and essential for differentiation of the endocrine pancreas Georg Mellitzer Georg Mellitzer Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Stefan Bonné Stefan Bonné Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Reini F Luco Reini F Luco Endocrinologia, Hospital Clinic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain Search for more papers by this author Mark Van De Casteele Mark Van De Casteele Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Nathalie Lenne-Samuel Nathalie Lenne-Samuel Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Patrick Collombat Patrick Collombat Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany Search for more papers by this author Ahmed Mansouri Ahmed Mansouri Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany Search for more papers by this author Jacqueline Lee Jacqueline Lee Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA Search for more papers by this author Michael Lan Michael Lan Department of Pediatrics, Children's Hospital, New Orleans, LA, USA Search for more papers by this author Daniel Pipeleers Daniel Pipeleers Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Finn C Nielsen Finn C Nielsen Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Search for more papers by this author Jorge Ferrer Jorge Ferrer Endocrinologia, Hospital Clinic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain Search for more papers by this author Gérard Gradwohl Corresponding Author Gérard Gradwohl Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Harry Heimberg Corresponding Author Harry Heimberg Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Georg Mellitzer Georg Mellitzer Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Stefan Bonné Stefan Bonné Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Reini F Luco Reini F Luco Endocrinologia, Hospital Clinic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain Search for more papers by this author Mark Van De Casteele Mark Van De Casteele Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Nathalie Lenne-Samuel Nathalie Lenne-Samuel Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Patrick Collombat Patrick Collombat Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany Search for more papers by this author Ahmed Mansouri Ahmed Mansouri Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany Search for more papers by this author Jacqueline Lee Jacqueline Lee Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA Search for more papers by this author Michael Lan Michael Lan Department of Pediatrics, Children's Hospital, New Orleans, LA, USA Search for more papers by this author Daniel Pipeleers Daniel Pipeleers Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Finn C Nielsen Finn C Nielsen Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark Search for more papers by this author Jorge Ferrer Jorge Ferrer Endocrinologia, Hospital Clinic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain Search for more papers by this author Gérard Gradwohl Corresponding Author Gérard Gradwohl Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France Search for more papers by this author Harry Heimberg Corresponding Author Harry Heimberg Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium Search for more papers by this author Author Information Georg Mellitzer1,‡, Stefan Bonné2,‡, Reini F Luco3, Mark Van De Casteele2, Nathalie Lenne-Samuel1, Patrick Collombat4, Ahmed Mansouri4, Jacqueline Lee5, Michael Lan6, Daniel Pipeleers2, Finn C Nielsen7, Jorge Ferrer3, Gérard Gradwohl 1 and Harry Heimberg 2 1Inserm' U682, Development and Physiopathology of the Intestine and Pancreas, Université Louis Pasteur, Strasbourg, France 2Diabetes Research Center, Vrije Universiteit Brussel, Brussels, Belgium 3Endocrinologia, Hospital Clinic de Barcelona, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Barcelona, Spain 4Department of Molecular Cell Biology, Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany 5Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO, USA 6Department of Pediatrics, Children's Hospital, New Orleans, LA, USA 7Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark ‡These authors contributed equally to this workInstitutions 1, 2, 3 and 7 are partners of the JDRF Center for Beta Cell Therapy in Diabetes; A Mansouri, J Lee, G Gradwohl and H Heimberg are members of the Beta Cell Biology Consortium *Corresponding authors: Diabetes Research Center, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium. Tel.: +32 2 477 4477; Fax: +32 2 477 4472; E-mail: [email protected] U682, Université Louis Pasteur, 3 avenue Moliere, 67200 Strasbourg, France. Tel.: +33 3 88 27 5366; Fax: +33 3 88 26 3538; E-mail: [email protected] The EMBO Journal (2006)25:1344-1352https://doi.org/10.1038/sj.emboj.7601011 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Neurogenin 3 (Ngn3) is key for endocrine cell specification in the embryonic pancreas and induction of a neuroendocrine cell differentiation program by misexpression in adult pancreatic duct cells. We identify the gene encoding IA1, a zinc-finger transcription factor, as a direct target of Ngn3 and show that it forms a novel branch in the Ngn3-dependent endocrinogenic transcription factor network. During embryonic development of the pancreas, IA1 and Ngn3 exhibit nearly identical spatio-temporal expression patterns. However, embryos lacking Ngn3 fail to express IA1 in the pancreas. Upon ectopic expression in adult pancreatic duct cells Ngn3 binds to chromatin in the IA1 promoter region and activates transcription. Consistent with this direct effect, IA1 expression is normal in embryos mutant for NeuroD1, Arx, Pax4 and Pax6, regulators operating downstream of Ngn3. IA1 is an effector of Ngn3 function as inhibition of IA1 expression in embryonic pancreas decreases the formation of insulin- and glucagon-positive cells by 40%, while its ectopic expression amplifies neuroendocrine cell differentiation by Ngn3 in adult duct cells. IA1 is therefore a novel Ngn3-regulated factor required for normal differentiation of pancreatic endocrine cells. Introduction The first morphological signs of the primitive pancreas emerge as dorsal and ventral protrusions of the primitive gut epithelium (Slack, 1995) at embryonic day (E) 9.5 in the mouse. Subsequently, all lineages defining the various pancreatic cell types, comprising endocrine islet and exocrine acinar and duct cells, are formed from a multipotent progenitor cell pool expressing the transcription factor Pdx1 (Gu et al, 2002). This process is regulated by a cascade of transcription factors that initiate and maintain the distinct genetic programs (Wilson et al, 2003; Jensen, 2004). The basic helix–loop–helix transcription factor Ngn3 is transiently expressed in a subset of the pancreas progenitor cells from E9.5 to E18.5 and initiates the differentiation program of all islet cells (Apelqvist et al, 1999; Gradwohl et al, 2000; Jensen et al, 2000; Schwitzgebel et al, 2000; Gu et al, 2002). Homozygous Ngn3-null mice thus fail to develop endocrine islet cells (Gradwohl et al, 2000) and premature or ectopic expression of Ngn3 in embryonic endoderm is sufficient to initiate endocrine cell differentiation (Apelqvist et al, 1999; Schwitzgebel et al, 2000; Grapin-Botton et al, 2001). The specification of different islet cell types and the completion of the differentiation process require the activation of transcription factors that are downstream of Ngn3. Of these regulatory factors NeuroD1, Pax4 and Nkx2.2 are direct targets of Ngn3 (Huang et al, 2000; Smith et al, 2003; Watada et al, 2003). Together with Nkx6.1 and Arx they act early in the differentiation/specification process (Sander et al, 2000; Collombat et al, 2003, 2005). Arx and Pax4 are required for the specification of the α- and β-cell lineage, respectively (Sosa-Pineda et al, 1997; Collombat et al, 2003, 2005). Further differentiation and maintenance of the endocrine phenotype depends on the activity of other transcription factors such as Isl1 and Pax6 (Ahlgren et al, 1997; Sander et al, 1997; St-Onge et al, 1997). Despite these findings, the precise genetic program controlling the differentiation of islet progenitors into beta cells remains unclear. Such knowledge is essential to generate functional beta cells in vitro for cell replacement therapy in type I diabetes. Ectopic expression of Ngn3 in adult human pancreatic duct cells supported this concept by activating the genes encoding Pdx1, NeuroD1, Pax4, Pax6, Nkx6.1 and Nkx2.2 and transdifferentiating duct cells into a β-cell-like phenotype, albeit with low levels of insulin (Heremans et al, 2002). In order to identify novel target genes of Ngn3, the transcriptome of adult human duct cells ectopically expressing Ngn3 was analysed on gene chips (Bonné et al, unpublished data). Among the genes most prominently induced by Ngn3 was that encoding the zinc-finger type transcription factor 'insulinoma associated 1' (IA1 or INSM1). IA1 was previously shown to be expressed in insulinoma and other endocrine tumours and cell lines, human embryonic pancreas and mouse nervous system (Goto et al, 1992; Lan et al, 1993; Zhu et al, 2002; Breslin et al, 2003; Pedersen et al, 2003). So far neither the expression pattern of IA1 nor its function during pancreas development have been addressed directly. The present study reveals that IA1 is transiently expressed in similar cells as Ngn3 during pancreatic development and shows that the IA1 gene is directly regulated by Ngn3 but not by other endocrine lineage transcription factors. It also provides evidence that IA1 ensures an essential stimulatory signal for proper formation of β- and α-cells. Results The zinc-finger transcription factor IA1 is a Ngn3 target Novel targets of the endocrinogenic master switch transcription factor Ngn3 were identified by analysis of the transcriptome of adult human pancreatic duct cells transduced with recombinant adenovirus expressing either Ngn3-GFP or GFP (Bonné et al, in preparation). IA1 (INSM1), a zinc-finger transcription factor in neoplastic β cells (Goto et al, 1992), was among the genes activated most strongly by Ngn3. Induction of IA1 preceded the expression of Pax4, NeuroD1 and Nkx2.2, which are direct targets of Ngn3 (Figure 1A) (Huang et al, 2000; Heremans et al, 2002; Smith et al, 2003; Watada et al, 2003) and essential for endocrinogenesis in the embryonic pancreas (Naya et al, 1997; Sosa-Pineda et al, 1997; Sussel et al, 1998). IA1 gene expression in adult duct cells becomes apparent at approximately 15–17 h following transduction, when the ectopic Ngn3 protein is first detected (Figure 1A and B). The appearance of IA1 mRNA in AdHANgn3-infected duct cells could be a consequence of Ngn3-induced activation of NeuroD1, since NeuroD1 is induced by Ngn3 (Huang et al, 2000) and because the proximal E3 box of the IA1 promoter is a reported target of the basic helix–loop–helix heterodimer NeuroD1/E47 (Breslin et al, 2003). However, IA1 was turned on by Ngn3 much earlier than NeuroD1 (Figure 1A) and IA1 transcripts were present at only low abundance 4 days following infection with AdNeuroD1 (Figure 1D). When expressed in adult human pancreatic duct cells at similar levels as Ngn3 or NeuroD1, other developmental transcription factors such as Foxa1, Foxa2, Pdx1, Pax4, Pax6, Nkx6.1 and Nkx2.2 failed to induce IA1 expression (Figure 1C). These data indicate that Ngn3 is a positive regulator of IA1, and this effect is unlikely to be mediated exclusively by NeuroD1 as an intermediary effector. Figure 1.Rapid and specific induction of IA1 expression in AdHANgn3-transduced adult human duct cells. (A) RT–PCR analysis of gene expression in AdHANgn3-transduced duct cells. Exogenous Ngn3 is detected at 4 h following transduction. IA1 expression is noticed at 17 h following transduction, which is earlier than the proposed direct Ngn3 target genes Pax4, NeuroD1 and Nkx2.2. (B) Exogenous HANgn3 protein is detected at 17 h post-transduction. Expression levels rise until 48 h post-transduction, after which they gradually drop and become undetectable at day 9. (C) Activation of IA1 gene expression in adult human pancreatic duct cells is observed following transduction with either AdHANgn3 and, albeit much weaker, AdNeuroD1, but not with adenoviral Foxa1, Foxa2, Pdx1, Pax4, Pax6, Nkx6.1, Nkx2.2 and GFP. RNA was extracted at 7 days following transduction. (D) Time course of the induction of IA1 expression in adult human duct cells transduced with either AdHANgn3 or AdNeuroD1. Induction of endogenous IA1 starts at 16 h post-infection with AdHANgn3 until at least 9 days following transduction. In contrast, induction of endogenous IA1 by AdNeuroD1 is obvious only at 4 days following transduction. Background signal caused by DNA of the single exon IA1 gene. NTC, nontransduced control. Download figure Download PowerPoint We next examined whether Ngn3 directly regulates the transcription of the IA1 gene. IA1 promoter-driven luciferase activity was increased in 293 cells when the reporter was cotransfected with a Ngn3 expression plasmid (Figure 2A). The extent of increase was similar to that observed in cells cotransfected with NeuroD1 and the reporter construct, in line with the observations by Breslin et al (2003). Supertransfection with cDNA encoding E47 did not influence the outcome of these experiments (data not shown). To establish whether Ngn3 binds directly to the IA1 gene promoter in vivo, chromatin immunoprecipitation was performed using adult human duct cells transduced with either AdHANgn3 or AdGFP. Genomic DNA was sheared to 200–1000 bp prior to immunoprecipitation (Figure 2B). Promoter regions of the IA1 and NeuroD1 genes but not control gene fragments from BRCA1 or CTLA4 were coimmunoprecipitated by HANgn3 (Figure 2C). Taken together, these results show that IA1 is a novel direct target of Ngn3, and hence becomes activated during the Ngn3-induced transdifferentiation program in adult human duct cells. Figure 2.Ngn3 binds and activates the IA1 promoter. (A) The IA1 promoter is activated by Ngn3 and NeuroD1 in transient promoter–reporter assays in 293 cells. Cotransfection of an IA1 promoter–reporter construct with Ngn3 (2) or NeuroD1 (3) increases IA1 promoter activity by twofold as compared to the IA1 promoter only (1). The pGL3basic (4) and pGL3control (5) samples represent negative and positive controls for promoter activity, respectively. All experiments were performed in triplicate and repeated at least three times. (B, C) Ngn3 interacts with the 5′ flanking regions of the IA1 and NeuroD1 genes. (B) Ethidium bromide-stained agarose gel showing chromatin sonicated to an average length of 200–1000 bp. *1000 bp; **500 bp. (C) Chromatin immunoprecipitation with an anti-HA antibody or IgG was performed on chromatin derived from isolated human duct cells infected with either HANgn3 or GFP. DNA from input chromatin was serially diluted as a reference for semiquantitative PCR analysis. The figures are representative of four independent experiments. The IA1 gene promoter is coimmunoprecipitated in the HANgn3-transduced duct cells using an anti-HA antibody but not by addition of IgG. Similarly, NeuroD1, but not BRCA1 or CTLA-4, are specifically co-precipitated by the anti-HA antibody. IA1, NeuroD1, BRCA1 and CTLA-4 are not precipitated in the negative control cells transduced with AdGFP. Download figure Download PowerPoint Transient expression of IA1 in islet progenitor cells of the mouse embryonic pancreas Since Ngn3 is not expressed in normal adult human or mouse pancreas (Gradwohl et al, 2000; Jensen et al, 2000; Schwitzgebel et al, 2000), our findings on Ngn3-dependent induction of IA1 prompted characterization of IA1 gene expression in embryonic mouse pancreas. At embryonic day E10.5, IA1 is detected in scattered cells (Figure 3A). Its highest expression levels are reached at E15.5 and subsequently decrease below detection limits from E18.5 (Figure 3A–D), correlating well with the timing of Ngn3 expression (Gradwohl et al, 2000). At embryonic day E15.5, IA1 transcripts were also observed in the duodenum and stomach, as well as in thymus, thyroid and adrenal glands (Figure 3E). In addition, IA1 is expressed in regions of the developing forebrain, midbrain and hindbrain, and the spinal cord (Figure 3E). In situ hybridization on adjacent cryo-sections showed that the spatio-temporal expression pattern of IA1 is similar to that of Ngn3 and partially overlapping with NeuroD1 (Figure 3F–H). In agreement with this observation, some cells express Ngn3 but not yet IA1, and IA1 transcripts appeared in undifferentiated cells that contain Ngn3 protein (Figure 3I). However, while no Ngn3-positive cells coexpressed islet hormones (Gradwohl et al, 2000), rare IA1-positive cells contained insulin or glucagon (Figure 3C, arrow), suggesting that the appearance of IA1 lags behind that of Ngn3 in the islet precursor cells. Furthermore, in the embryonic pancreas rare IA1-expressing cells are actively cycling, as demonstrated by BrdU incorporation (Figure 3J). Figure 3.Overlapping expression patterns of IA1 and Ngn3 in islet progenitor cells of mouse embryo. (A) IA1 gene transcription in the pancreas starts at E10.5, as shown by in situ hybridization. Dashed line delimits the pancreatic epithelium. (B, C) Insulin and glucagon are present in few IA1-expressing cells (arrow in C). (D) The number of IA1-expressing cells increases until E15.5, then rapidly decreases and no IA1 expression can be detected at E18.5. (E) In E15.5, mouse IA1 transcripts (blue staining) are observed in the developing nervous system and endocrine glands as well as in the pancreas and gastrointestinal tract. (F–H) In situ hybridization on consecutive pancreas sections shows coexpression of IA1 and Ngn3 in islet precursor cells, while coexpression with NeuroD1 is limited. (I) IA1 (in situ hybridization, blue) and Ngn3 (immunohistochemistry, brown) are coexpressed in endocrine progenitor cells (arrows). (J) BrdU labels IA1-expressing cells (arrows). ad, adrenal gland; fbr, forebrain; hbr, hindbrain; int, intestine; mbr, midbrain; panc, pancreas; thym, thymus; thyr, thyroid; sto, stomach. Magnification A–D and F–H: × 40, E: × 5, I and J: × 63. Download figure Download PowerPoint IA1 expression is selective in the endocrine lineage and depends on Ngn3 To determine conclusively whether IA1 is expressed selectively in islet progenitors or also in immature acinar or duct cells, IA1 expression was examined in Ngn3 knockout mice that lack all types of islet cells (Gradwohl et al, 2000). No IA1-expressing cells were detected in pancreas of the Ngn3 null mutants (Figure 4A and B), ascertaining that IA1 is exclusively expressed in the endocrine lineage of the embryonic pancreas. Furthermore, it supports that Ngn3 is necessary to induce IA1 not only in transdifferentiating adult duct cells in vitro but also in embryonic progenitor cells in vivo. Figure 4.IA1 is specifically expressed in the islet lineage, immediately downstream of the proendocrine gene Ngn3. (A–J) In situ hybridization with probes specific for IA1 and Ngn3 on consecutive cryosections of embryonic pancreas from mutant mice deficient for the Ngn3, NeuroD1, Pax4, Arx or Pax6 transcription factors, respectively. (A, B) No IA1 transcripts are detected in the pancreas of Ngn3-deficient mice. Expression of IA1 and Ngn3 is unaffected in the pancreas of NeuroD1 (C, D), Pax4 (E,F), Arx (G, H) and Pax6 (I, J) null mutant embryos. Signals for IA1 and Ngn3 overlap on consecutive sections of pancreas from the different mutant mice. Magnification A–J: × 40. Download figure Download PowerPoint As mentioned earlier, data obtained by others (Breslin et al, 2003) and ourselves (Figure 1C and D) indicate that NeuroD1 may also activate IA1 transcription. We therefore analysed the expression pattern of IA1 and Ngn3 on adjacent cryosections of pancreas from mouse embryos that were null mutant for NeuroD1 (Figure 4C and D). We also assessed embryos deficient for other key developmental transcription factors acting downstream of Ngn3, namely Pax4, Arx and Pax6 (Figure 4E–J) (Sosa-Pineda et al, 1997; St-Onge et al, 1997; Huang et al, 2000; Collombat et al, 2003, 2005). IA1 and Ngn3 have a similar expression pattern both in NeuroD1 mutant and wild-type embryonic pancreas (Figure 4C and D versus Figure 3F and G). This observation demonstrates that NeuroD1 is not essential for the expression of both IA1 and Ngn3 (Figure 4C and D). In addition, the expression of mRNAs encoding IA1 or Ngn3 is also not affected in the islet precursors of Pax4−/− (Figure 4E and F), Arx−/− (Figure 4G and H) or Pax6−/− (Figure 4I and J) mutant mice. These results show that IA1 is exclusively expressed in the Ngn3-dependent cellular lineage and that its expression is dependent on Ngn3 but not on known downstream regulators. Together with data indicating that Ngn3 occupies the IA1 gene, these findings place IA1 at a discrete early step subsequent to Ngn3 in the regulatory cascade that drives pancreatic endocrine differentiation. IA1 is an essential regulator of endocrine cell generation in the embryonic mouse pancreas To further clarify the role of IA1 in pancreatic endocrine development, tissue explants from E12.5 pancreas were cultured under conditions that allow endocrine cell differentiation and islet cell formation (Miralles et al, 1998; Mellitzer et al, 2004). The pancreatic rudiments were isolated from mice expressing the enhanced green fluorescent protein (eGFP) as a reporter under control of the Ngn3 promoter and thus enable tracing of endocrine progenitor cells (data not shown). Explants were incubated with IA1-specific antisense oligonucleotides of the morpholino-type to inactivate IA1 translation. Although antisense oligonucleotide technology to knockdown specific gene expression in embryonic pancreas in vitro has been reported before (Prasadan et al, 2002; Li et al, 2004), we validated the uptake of biotinylated morpholinos by immunostaining (Figure 5B and B′) and observed a gradient towards the inside of the explant. Two antisense oligonucleotides of the morpholino-type were designed (sequence under Materials and methods) to target different segments of the IA1 mRNA sequence. Antisense or control oligonucleotides were incubated with the pancreatic explants during the entire period of culture. As compared to nontreated explant cultures, presence of the control morpholino oligonucleotides (Figure 5A–E) did not alter growth rate (unpublished data) or normal cell differentiation. However, while differentiation of exocrine cells was not affected by the antisense morpholinos, as shown by immunostaining of amylase-positive cells (Figure 5E′), both anti-IA1 oligos reduced the total number of cells containing immunoreactive glucagon or insulin to a similar extent, that is, 40% (Figure 5D′ and F). Figure 5.Inhibition of IA1 translation blocks islets cell differentiation. E12.5 dorsal pancreatic epithelia were isolated from Ngn3-promoter-driven eGFP transgenic embryos, the surrounding mesenchyme was dissected away and the remaining epithelial cells were cultured for 4 days in the presence of standard missense control morpholinos (A–E) or IA1-specific morpholinos (A′–E′). (A, A′) Bright field image showing normal growth of pancreatic rudiments. (B, B′) Peroxidase staining (brown) shows the uptake and distribution of biotinylated antisense oligonucleotides in the pancreatic rudiments. (C, C′) eGFP fluorescence of Ngn3+ endocrine progenitor cells in living explants. (D, D′) eGFP fluorescence combined with immunodetection of insulin and glucagon (red) on cryosections of pancreatic explants at day 4 of culture. Cell nuclei are counterstained with DAPI. (E, E′) Immunodetection of amylase (red) on cryosections of pancreatic explants at day 4 of culture. Cell nuclei are counterstained with DAPI. Magnification A–E: × 20. (F) Quantification of Ngn3-eGFP and insulin- and glucagon-expressing cells in standard missense control-treated or IA1 antisense-treated pancreatic explants shows a significant decrease (morpholino 1 P<0.02, morpholino 2 P<0.05) in islet cell numbers in IA1 antisense-treated explants. Each figure represents the mean±s.e.m. of four (morpholino 1) or three (morpholino 2) independent experiments with 3–4 explants per individual experiment. Statistical significance of the data was determined by unpaired, two-tailed Student's t-test. Download figure Download PowerPoint IA1 enhances transdifferentiation of adult human pancreatic duct cells As ectopic expression of Ngn3 recapitulates embryonic endocrinogenesis in adult pancreatic duct cells (Heremans et al, 2002), the capacity of the direct Ngn3 target IA1 to induce or improve duct cell transdifferentiation was investigated. Ectopic IA1 alone did not activate the genes encoding the developmental transcription factors NeuroD1, Pax4 and Nkx2.2, Delta-Notch signalling cascade components DLL1, DLL4, Hes6 and Hes1, or neuroendocrine marker genes such as synaptophysin (SYP), chromogranin A (CHGA), PC1/3 and INS (Figure 6A). As IA1 was originally characterized as a transcriptional repressor (Breslin et al, 2002), its interaction with Ngn3-induced gene expression mediating endocrine cell development or function was analysed by normal (Figure 6B) and real time (Figure 6C) RT–PCR. Coexpression of Ngn3 and IA1 in duct cells enhanced the induction of the Ngn3 target genes NeuroD1, Pax4 and Nkx2.2 by 2–6-fold depending on the target gene (n=3, P<0.05) (Figure 6C). These observations thus suggest that IA1 and Ngn3 act in concert to stimulate the endocrinogenic program in the pancreas. Figure 6.Profiling gene expression in adult human pancreatic duct cells following ectopic expression of Ngn3 and IA1. (A) Duct cells were transduced with either AdHANgn3, AdIA1 or AdGFP and processed for RT–PCR analysis at 7 days following transduction. Exogenous HANgn3, IA1 and Ngn3-induced endogenous IA1 are readily detected in AdIA1 and AdHANgn3 samples, respectively, but not in the AdGFP control. Viral Ngn3 contains a HA-tag and is detected in the AdHANgn3 samples only. Ectopic IA1 expression does not induce endogenous NeuroD1, Pax4 or Nkx2.2 transcription factor expression, in contrast to Ngn3. Induction of Delta-Notch signaling components DLL1, DLL4 and Hes6 by Ngn3 is not recapitulated by IA1, but remains at expression levels comparable to the GFP control sample. IA1 does not influence Hes1 mRNA abundance. The endocrine marker genes encoding SYP, CHGA, prohormone convertase 1/3 (PC1/3) and, albeit to a lesser extent, insulin (INS) are induced by ectopic Ngn3 expression but remain similar to control levels in IA1-expressing cells. Control RT–PCRs detect transcripts for GFP and GAPD. (B) Cotransduction of Ngn3 and IA1 enhances Ngn3-mediated transdifferentiation in adult human duct cells. Cells were transduced with AdHANgn3 and AdGFP, AdHANgn3 and AdIA1 or AdGFP only at a constant total MOI of 110, and processed for RT–PCR analysis at 3 days following transduction. Exogenous HANgn3, IA1 and Ngn3-induced endogenous IA1 are readily detected. Expression of the Ngn3 target genes NeuroD1, Pax4 and Nkx2.2 is enhanced in the AdHANgn3 and AdIA1 cotransduced samples as compared to the AdHANgn3 sample. RT–PCR results also indicate higher expression of the Delta-Notch component Hes6 and the endocrine marker SYP in Ngn3 and IA1 cotransduced cells. A control GAPD RT–PCR indicates comparable amounts of cDNA input. (C) The level of transcripts shown in (B) was quantified by real-time RT–PCR using specific Taqman probes as described in 'Materials and methods' and revealed a 2–6-fold increase in target mRNA following cotransduction of adult duct cells with Ngn3 and IA1 as compared to transduction with Ngn3 alone (n=3). Statistical significance of the data was determined b
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