Portal de Periódicos da CAPES

Ultradeep Pyrosequencing Detects Complex Patterns of CD8 + T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques

2009; American Society for Microbiology; Volume: 83; Issue: 16 Linguagem: Inglês

10.1128/jvi.00897-09

1098-5514

Benjamin N. Bimber, Benjamin J. Burwitz, Shelby L. O’Connor, Ann Detmer, Emma Gostick, Simon M. Lank, David A. Price, Austin L. Hughes, David H. O’Connor,

Mosquito-borne diseases and control

ABSTRACT Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8 + T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.