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Application Of FTIR Spectroscopy For A Rapid Determination Of Some Hydrolytic Enzymes Activity On Sea Buckthorn Substrate

2010; University of Bucharest; Volume: 15; Issue: 6 Linguagem: Inglês

2248-3942

Florinela Fetea, Abdelmoumen Taoutaou, Carmen Socaciu, Département de Botanique, Carmen Socaciu,

Food Industry and Aquatic Biology

We investigated the efficiency of some commercial cellulosic and pectinic enzymes (Carezyme 1000 L and Pectinex Ultra SP-L, with cellulose, pectinase and hemicellulase activity, respectively) on a sea buckthorn puree substrate. The method applied to check the rate of hydrolysis was Fourier Transformed Infrared Spectroscopy (FTIR), by recording the absorption of different carbohydrates in the specific spectral range (650-4000 cm -1 ). To calibrate the method, we used firstly, different concentrations of pure glucose, fructose and sucrose (from 1 to 25 %) and registered their IR maximal wavenumbers and peak intensity. Based on calibration curves, we calculated the release in vitro of glucose, fructose or sucrose after enzymatic action on sea buckthorn substrate. According to the IR absorption peaks registered for glucose (at 1033 cm -1 ), fructose (at 1063 cm -1 ) and sucrose (at 995 cm - 1 ), we were able to identify the changes in the shape and intensity of these peaks in the region 1200-900 cm -1 , against control. FTIR spectrometry proved to be a suitable method for accurate and direct determination of individual sugars (glucose, fructose and sucrose - like disaccharides) on sea buckthorn or other juices and a good control of enzymatic activity on cellulose, hemicellulose and pectine-rich substrates. The time used for FTIR analysis is considerably reduced compared to the classical methods. These results are promising and have a real analytical potential to supervise in situ or in vitro, as a routine procedure, the dynamics of enzymatic treatment of natural substrates rich in natural carbohydrate polymers (celluloses and pectins).