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HapMap SNP Scanner: an online program to mine SNPs responsible for cell phenotype

2012; Wiley; Volume: 80; Issue: 2 Linguagem: Inglês

10.1111/j.1399-0039.2012.01883.x

1399-0039

T. Yamamura, J. Hikita, Marie Bleakley, Tomoya Hirosawa, Aiko Sato‐Otsubo, Hiroki Torikai, Takashi Hamajima, Yasuhito Nannya, Ayako Demachi‐Okamura, Etsuko Maruya, H. Saji, Yukiya Yamamoto, Takashi Takahashi, Nobuhiko Emi, Y Morishima, Y Kodera, Kiyotaka Kuzushima, Stanley R. Riddell, Seishi Ogawa, Yoshiki Akatsuka,

Immunotherapy and Immune Responses

Minor histocompatibility (H) antigens are targets of graft‐ vs ‐host disease and graft‐ vs ‐tumor responses after human leukocyte antigen matched allogeneic hematopoietic stem cell transplantation. Recently, we reported a strategy for genetic mapping of linkage disequilibrium blocks that encoded novel minor H antigens using the large dataset from the International HapMap Project combined with conventional immunologic assays to assess recognition of HapMap B‐lymphoid cell line by minor H antigen‐specific T cells. In this study, we have constructed and provide an online interactive program and demonstrate its utility for searching for single‐nucleotide polymorphisms (SNPs) responsible for minor H antigen generation. The website is available as ‘HapMap SNP Scanner’, and can incorporate T‐cell recognition and other data with genotyping datasets from CEU, JPT, CHB, and YRI to provide a list of candidate SNPs that correlate with observed phenotypes. This method should substantially facilitate discovery of novel SNPs responsible for minor H antigens and be applicable for assaying of other specific cell phenotypes (e.g. drug sensitivity) to identify individuals who may benefit from SNP‐based customized therapies.