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Increased production of endothelin‐1 in patients with inflammatory arthritides

1992; Wiley; Volume: 35; Issue: 4 Linguagem: Inglês

10.1002/art.1780350406

1529-0131

Nobuyuki Miyasaka, Yukio Hirata, Kenji Andò, Kazuto Sato, H. Morita, Masayoshi Shichiri, Kazuo Kanno, Kimio Tomita, Fumiaki Marumo,

Asthma and respiratory diseases

Abstract Objective. Endothelin‐1 (ET‐1) is a potent vasoconstrictive peptide, but its precise mechanism of action in vivo has remained unknown. Methods. We measured ET‐1 activity by radioimmunoassay, in both plasma and synovial fluid from patients with inflammatory arthritides. Results. ET‐1–like immunoreactivity was found in synovial fluid, at levels severalfold higher than those in plasma. Reverse‐phase high‐performance liquid chromatography showed an elution profile corresponding to actual ET‐1. A single class of high‐affinity binding sites for ET‐1 in cultured synovial cells was also detected. Furthermore, ET‐1 induced mild DNA synthesis in synovial cells. Conclusion. Taken together, these results indicate that ET‐1 might contribute to the synovial proliferation seen in inflammatory arthritides, in an autocrine/paracrine manner.