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Natronoarchaeum mannanilyticum gen. nov., sp. nov., an aerobic, extremely halophilic archaeon isolated from commercial salt

2009; Microbiology Society; Volume: 60; Issue: 11 Linguagem: Inglês

10.1099/ijs.0.016600-0

1466-5034

Yasuhiro Shimane, Yuji Hatada, Hiroaki Minegishi, Toru Mizuki, Akinobu Echigo, Masayuki Miyazaki, Yukari Ohta, Ron Usami, William D. Grant, Koki Horikoshi,

Diatoms and Algae Research

Strain YSM-123 T was isolated from commercial salt made from Japanese seawater in Niigata prefecture. Optimal NaCl and Mg 2+ concentrations for growth were 4.0–4.5 M and 5 mM, respectively. The isolate was a mesophilic and slightly alkaliphilic haloarchaeon, whose optimal growth temperature and pH were 37 °C and pH 8.0–9.0. Phylogenetic analysis based on 16S rRNA gene sequence analysis suggested that strain YSM-123 T is a member of the phylogenetic group defined by the family Halobacteriaceae , but there were low similarities to type strains of other genera of this family (≤90 %); for example, Halococcus (similarity <89 %), Halostagnicola (<89 %), Natronolimnobius (<89 %), Halobiforma (<90 %), Haloterrigena (<90 %), Halovivax (<90 %), Natrialba (<90 %), Natronobacterium (<90 %) and Natronococcus (<90 %). The G+C content of the DNA was 63 mol%. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, disulfated diglycosyl diether and an unknown glycolipid. On the basis of the data presented, we propose that strain YSM-123 T should be placed in a new genus and species, Natronoarchaeum mannanilyticum gen. nov., sp. nov. The type strain of Natronoarchaeum mannanilyticum is strain YSM-123 T (=JCM 16328 T =CECT 7565 T ).