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The use of hypobromite oxidation to evaluate two current methods for the estimation of inositol polyphosphates in alkaline extracts of soils

1981; Taylor & Francis; Volume: 12; Issue: 5 Linguagem: Inglês

10.1080/00103628109367169

1532-2416

G.C.J. Irving, Daniel J. Cosgrove,

Cassava research and cyanide

Abstract Two current methods (McKercher and Anderson and Steward and Tate18) for the estimation of inositol polyphosphates in soil extracts were examined for accuracy by subjecting the inositol polyphosphate fraction obtained by each method to oxidation with alkaline hypobromite. Anion‐exchange chromatography of the resistant phosphate showed that with both methods a large proportion of the organic phosphorus in the fraction was neither inositol pentakisphosphate nor inositol hexakisphosphate. The results of methods for the estimation of these compounds in soil extracts which do not include an oxidation with alkaline hypobromite must therefore be suspect.