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High-throughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance

2012; National Academy of Sciences; Volume: 109; Issue: 28 Linguagem: Inglês

10.1073/pnas.1208003109

1091-6490

Rebecca Bart, Megan Cohn, Amirkhan Kassen, Emily J. McCallum, Mikel Shybut, Annalise Petriello, Ksenia V. Krasileva, Douglas Dahlbeck, Cesar Augusto Medina, Titus Alicai, P. Lava Kumar, Leandro Márcio Moreira, Júlio Rodrigues Neto, Valérie Verdier, Marı́a Angélica Santana, N. Kositcharoenkul, Hervé Vanderschuren, Wilhelm Gruissem, Adriana Bernal, Brian J. Staskawicz,

Nematode management and characterization studies

Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis ( Xam ), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies.