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The infection of primary avian tracheal epithelial cells with infectious bronchitis virus

2009; BioMed Central; Volume: 41; Issue: 1 Linguagem: Inglês

10.1051/vetres/2009054

1297-9716

Ching‐I Shen, Ching‐Ho Wang, Jiunn‐Wang Liao, Tien-Wang Hsu, Shu-Ming Kuo, Hong-Lin Su,

Viral gastroenteritis research and epidemiology

Here we introduce a culture system for the isolation, passaging and amplification of avian tracheal epithelial (ATE) cells.The ATE medium, which contains chicken embryo extract and fetal bovine serum, supports the growth of ciliated cells, goblet cells and basal cells from chicken tracheas on fibronectin-or matrigel-coated dishes.Non-epithelial cells make up less than 10% of the total population.We further show that ATE cells support the replication and spread of infectious bronchitis virus (IBV).Interestingly, immunocytostaining revealed that basal cells are resistant to IBV infection.We also demonstrate that glycosaminoglycan had no effect on infection of the cells by IBV.Taken together, these findings suggest that primary ATE cells provide a novel cell culture system for the amplification of IBV and the in vitro characterization of viral cytopathogenesis.tracheal epithelial cell / infectious bronchitis virus / basal cell / primary culture /