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Reactive microgliosis engages distinct responses by microglial subpopulations after minor central nervous system injury

2005; Wiley; Volume: 82; Issue: 4 Linguagem: Inglês

10.1002/jnr.20659

1097-4547

Martin Wirenfeldt, Alicia A. Babcock, Rune Ladeby, Kate Lykke Lambertsen, Frederik Dagnæs‐Hansen, Robert Graham Quinton Leslie, Trevor Owens, Bente Finsen,

Neurogenesis and neuroplasticity mechanisms

Journal of Neuroscience ResearchVolume 82, Issue 4 p. 507-514 Research Article Reactive microgliosis engages distinct responses by microglial subpopulations after minor central nervous system injury Martin Wirenfeldt, Corresponding Author Martin Wirenfeldt [email protected] Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark The first two authors contributed equally to this work.Medical Biotechnology Center, University of Southern Denmark, Winsløwparken 25, DK-5000 Odense C, DenmarkSearch for more papers by this authorAlicia Anne Babcock, Alicia Anne Babcock Neuroimmunology Unit, Montreal Neurological Institute, Montreal, Quebec, Canada The first two authors contributed equally to this work.Search for more papers by this authorRune Ladeby, Rune Ladeby Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorKate Lykke Lambertsen, Kate Lykke Lambertsen Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorFrederik Dagnaes-Hansen, Frederik Dagnaes-Hansen Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, DenmarkSearch for more papers by this authorRobert Graham Quinton Leslie, Robert Graham Quinton Leslie Immunology and Microbiology, Institute of Medical Biology, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorTrevor Owens, Trevor Owens Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark Neuroimmunology Unit, Montreal Neurological Institute, Montreal, Quebec, CanadaSearch for more papers by this authorBente Finsen, Bente Finsen Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this author Martin Wirenfeldt, Corresponding Author Martin Wirenfeldt [email protected] Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark The first two authors contributed equally to this work.Medical Biotechnology Center, University of Southern Denmark, Winsløwparken 25, DK-5000 Odense C, DenmarkSearch for more papers by this authorAlicia Anne Babcock, Alicia Anne Babcock Neuroimmunology Unit, Montreal Neurological Institute, Montreal, Quebec, Canada The first two authors contributed equally to this work.Search for more papers by this authorRune Ladeby, Rune Ladeby Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorKate Lykke Lambertsen, Kate Lykke Lambertsen Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorFrederik Dagnaes-Hansen, Frederik Dagnaes-Hansen Department of Medical Microbiology and Immunology, University of Aarhus, Aarhus, DenmarkSearch for more papers by this authorRobert Graham Quinton Leslie, Robert Graham Quinton Leslie Immunology and Microbiology, Institute of Medical Biology, University of Southern Denmark, Odense, DenmarkSearch for more papers by this authorTrevor Owens, Trevor Owens Medical Biotechnology Center, University of Southern Denmark, Odense, Denmark Neuroimmunology Unit, Montreal Neurological Institute, Montreal, Quebec, CanadaSearch for more papers by this authorBente Finsen, Bente Finsen Medical Biotechnology Center, University of Southern Denmark, Odense, DenmarkSearch for more papers by this author First published: 19 October 2005 https://doi.org/10.1002/jnr.20659Citations: 45 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Microglia are bone marrow-derived cells that constitute a facultative macrophage population when activated by trauma or pathology in the CNS. Endogenous CNS-resident microglia as well as exogenous (immigrant) bone marrow-derived cells contribute to reactive microgliosis, raising fundamental questions about the cellular composition, kinetics, and functional characteristics of the reactive microglial cell population. Bone marrow chimeric mice reconstituted with green fluorescent protein-expressing (GFP+) donor bone marrow cells were subjected to entorhinal cortex lesion, resulting in selective axonal degeneration and a localized microglial reaction in the hippocampus. Flow cytometric evaluation of individually dissected hippocampi differentiated immigrant GFP+ microglia from resident GFP− microglia (CD11b+CD45dim) and identified a subset of mainly resident CD11b+ microglia that was induced to express CD34. The proportion of immigrant GFP+ microglia (CD11b+CD45dim) increased signficantly by 3 and 5 days postlesion and reached a maximum of 13% by 7 days. These cells expressed lower CD11b levels than resident microglia, forming a distinct subpopulation on CD11b/CD45 profiles. The proportion of CD34+CD11b+ microglia was significantly increased at 3 days postlesion but had normalized by 5 and 7 days, when the microglial reaction is known to be at its maximum. Our results show that distinct subpopulations of microglia respond to minor CNS injury. The heterogeneity in microglial response may have functional consequences for repair and possibly therapy. © 2005 Wiley-Liss, Inc. REFERENCES Aloisi F. 2001. Immune function of microglia. Glia 36: 165–179. 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