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Re-organization of mitochondria at the NK cell immune synapse

2008; Elsevier BV; Volume: 122; Issue: 1 Linguagem: Inglês

10.1016/j.imlet.2008.10.008

1879-0542

Edgar Abarca‐Rojano, Saé Muñiz‐Hernández, María Maximina Bertha Moreno‐Altamirano, Ricardo Mondragón‐Flores, Fernando Enrı́quez-Rincón, Francisco Javier Sánchez‐García,

Endoplasmic Reticulum Stress and Disease

As part of the innate immune response NK cells destroy infected, transformed, or otherwise stressed cells within hours of activation. In contrast, CD4+ T lymphocytes require a sustained increase in their metabolism in order to cope with the biogenesis of cell components, in a process of proliferation and differentiation into effector cells. Recently, mitochondria have been implied in T lymphocyte immune synapse function but little is known on the role of mitochondria in the NK cell interaction with tumour cells. Here we analysed NK cells mitochondrial membrane potential (Δψm) as an indicator of mitochondrial energy status and cellular homeostasis. Upon contact with K562 tumour cells, NK cells undergo Δψm depolarization, indicating a rapid consumption of their metabolic energy. Furthermore, pharmacological inhibition of ATP synthesis down-regulates NK cell cytotoxic activity. Confocal- and electron-microscopy analyses showed re-organization of NK cells mitochondria towards the site of interaction with K562 tumour cell (NK cell immune synapse), perhaps as a way to compensate for local energy consumption. Interestingly, mitochondrial re-organization also takes place following NK stimulation with anti-NKGD2 antibodies but not with anti-KIR2DL1 antibodies, suggesting that activating rather than inhibiting cell signalling, triggered by NK cell receptors, is involved in NK cell mitochondria dynamics.