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Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

2010; Wiley; Volume: 26; Issue: 5 Linguagem: Inglês

10.1111/j.1439-0426.2010.01544.x

1439-0426

P. Li, Marek Rodina, Martin Hulák, David Gela, Z. H. Li, Otomar Linhart,

Sperm and Testicular Function

Journal of Applied IchthyologyVolume 26, Issue 5 p. 753-755 Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso P. Li, P. Li University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorM. Rodina, M. Rodina University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorM. Hulak, M. Hulak University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorD. Gela, D. Gela University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorZ. H. Li, Z. H. Li University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorO. Linhart, O. Linhart University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this author P. Li, P. Li University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorM. Rodina, M. Rodina University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorM. Hulak, M. Hulak University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorD. Gela, D. Gela University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this authorZ. H. Li, Z. H. Li University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, ChinaSearch for more papers by this authorO. Linhart, O. Linhart University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech RepublicSearch for more papers by this author First published: 17 September 2010 https://doi.org/10.1111/j.1439-0426.2010.01544.xCitations: 10 Author's address: P. Li, University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, 728/II Zatisi, 389 25 Vodnany, Czech Republic; Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China.E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Summary Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 μms−1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml−1), spermatozoa concentration (0.28 ± 0.27 × 109 spz ml−1), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg−1), the pH (8.49 ± 0.01) and Na+ (18.97 ± 3.65 mm), K+ (2.83 ± 1.36 mm), Ca2+ (0.19 ± 0.06 mm), Mg2+ (0.49 ± 0.23 mm) and Cl− (6.33 ± 0.58 mm) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and iso-electric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon. References Billard, R.; Cosson, J.; Fierville, F.; Brun, R.; Rouault, T.; Williot, P., 1999: Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa. J. Appl. Ichthyol. 15, 199– 203. Birstein, V. J.; DeSalle, R., 1998: Molecular phylogeny of Acipenserinae. Mol. Phylogenet. Evol. 9, 141– 155. Caille, N.; Rodina, M.; Kocour, M.; Gela, D.; Flajshans, M.; Linhart, O., 2006: Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments. Aqua. Int. 14, 75– 87. Ciereszko, A.; Dabrowski, K.; Lin, F.; Doroshov, S. I., 1994: Identification of trypsin-like activity in sturgeon spermatozoa. J. Exp. Zool. 268, 486– 491. Cosson, J.; Linhart, O.; Mims, S. D.; Shelton, W. 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Asturiano, Otomar Linhart, Akos HorvathOctober 2010Pages 753-755 ReferencesRelatedInformation