Constitutive and Inducible Green Fluorescent Protein Expression in Bartonella henselae
1998; American Society for Microbiology; Volume: 66; Issue: 8 Linguagem: Inglês
10.1128/iai.66.8.3964-3967.1998
ISSN1098-5522
AutoresAnthea K. Lee, Stanley Falkow,
Tópico(s)Bacterial Identification and Susceptibility Testing
ResumoABSTRACT The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae , and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37°C was isolated.
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