Portal de Periódicos da CAPES

Fase intestinal de Trichinella spiralis en modelo murino

2009; National University of Colombia; Volume: 14; Issue: 1 Linguagem: Espanhol

1900-1649

María Alejandra Moreno García, Claudia Herminia Maldonado Tapia, Elda Araceli García Mayorga, Rosa Gabriela Reveles Hernández, José Jesús Muñoz Escobedo,

Parasitic Diseases Research and Treatment

RESUMEN Trichinella spiralis se encuentra principalmente en el hombre, rata, cerdo, perro; puede infectar a cualquier carnivoro u omnivoro. Cuando la carne o sus derivados estan contaminados con larvas infectantes (LI) de T. spiralis y son ingeridas estas pasan al estomago, donde sus capsulas son disueltas por accion de los jugos gastricos, las larvas son liberadas en pocas horas, despues pasan a la porcion proximal del intestino delgado, donde se lleva a cabo su desarrollo. El objetivo del presente trabajo fue evaluar la fase intestinal de T. spiralis en un modelo murino. Un lote de 45 ratas Long Evans, se infectaron con aproximadamente 500 LI, y fueron sacrificadas tres diarias por 15 dias. Se tomo un segmento de duodeno, yeyuno e ileon y se fijaron en formol al 10% para posteriormente ser procesados en parafina y tenido con hematoxilina-eosina. El resto del intestino delgado fue fraccionado, se incubo a 37 oC por dos horas y el sobrenadante se observo al microscopio de luz. Se encontro que el implante se lleva a cabo a nivel de yeyuno e ileon, que las hembras adultas dan origen aproximadamente 60-80 larvas recien nacidas (LRN), parto viviparo en un tercio distal y subse-cuentemente son destruidas. Los machos adultos tienen espermatozoides no ciliados. Palabras clave: larvas infectantes, adultos, larvas recien nacidas. ABSTRACT Trichinella spiralis is a parasitic disease in man, rat, pig, but can infect any carnivorous or omnivorous. When the meat or their derivates are contaminated with infective larvae (il) of T. spiralis pass to the stomach, their capsules are dissolved by the stomach juice, the larvaes are liberated in few hours, and then they pass to the near portion of the slim intestine in which they develop. The objective of the present work was to evaluate the intestine phase of T. spiralis in a murine model. Fourtyfive Long Evans rats were infected with 500 li approximately, then 3 rats were sacrificed everyday over a period of 15 days. A portion of duodenum, jejune and ileum were fixed in formaldehyde 10% and subsequently embeded in paraffin and dyed with hematoxilin-eosina. The rest of the slim intestine was cut and fractionated and incubated at 37 oc for 2 hours; the supernadant was observed unde light microscopy. We observed that the implant of larvae was in the jejune and ileum. The adult females gave origin to approximately 6080 newborn larvaes (LRN) and subsequently were destroyed. The adult male had non-ciliated sperm. Key words: Infectant larvae, adult, recently born larvae.