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Cofactor Trapping, a New Method To Produce Flavin Mononucleotide

2010; American Society for Microbiology; Volume: 77; Issue: 3 Linguagem: Inglês

10.1128/aem.01541-10

1098-5336

Ulrich Krauß, Vera Svensson, Astrid Wirtz, Esther Knieps‐Grünhagen, Karl‐Erich Jaeger,

Microbial Metabolic Engineering and Bioproduction

ABSTRACT We have purified flavin mononucleotide (FMN) from a flavoprotein-overexpressing Escherichia coli strain by cofactor trapping. This approach uses an overexpressed flavoprotein to trap FMN, which is thus removed from the cascade regulating FMN production in E. coli . This, in turn, allows the isolation of highly pure FMN.