Artigo Acesso aberto Produção Nacional Revisado por pares

Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus

2012; Springer Science+Business Media; Volume: 157; Issue: 10 Linguagem: Inglês

10.1007/s00705-012-1384-x

ISSN

1432-8798

Autores

Álvaro Júlio Pereira, Poliane Alfenas‐Zerbini, Renan de Souza Cascardo, E. C. de Andrade, F. Murilo Zerbini,

Tópico(s)

Bacteriophages and microbial interactions

Resumo

Papaya lethal yellowing virus (PLYV) causes an economically important disease in papayas in northeastern Brazil. Based on biological and molecular properties, PLYV has been tentatively assigned to the genus Sobemovirus. We report the sequence of the full-length genome of a PLYV isolate from Brazil, determined by deep sequencing. The PLYV genome is 4,145 nt long and contains four ORFs, with an arrangement identical to that of sobemoviruses. The polyprotein and CP display significant sequence identity with the corresponding proteins of other sobemoviruses. Pairwise comparisons and phylogenetic analysis based on complete nucleotide sequences confirm the classification of PLYV in the genus Sobemovirus.

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