Portal de Periódicos da CAPES

Fluoromorphic Substrates for Fatty Acid Metabolism: Highly Sensitive Probes for Mammalian Medium‐Chain Acyl‐CoA Dehydrogenase

2005; Wiley; Volume: 45; Issue: 4 Linguagem: Inglês

10.1002/anie.200502675

1521-3773

Mary K. Froemming, Dalibor Sameš,

Adipose Tissue and Metabolism

Not fussy: A systematic study revealed considerable substrate tolerance of medium-chain acyl-CoA dehydrogenase (MCAD), a member of the β-oxidation pathway for the degradation of fatty acids. Three fluorogenic reporter substrates were developed, which in turn allowed for selective, sensitive, and continuous monitoring of MCAD activity in tissue homogenates. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z502675_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.