Portal de Periódicos da CAPES

Global Transposon Mutagenesis and a Minimal Mycoplasma Genome

1999; American Association for the Advancement of Science; Volume: 286; Issue: 5447 Linguagem: Inglês

10.1126/science.286.5447.2165

1095-9203

Clyde A. Hutchison, Scott N. Peterson, Steven R. Gill, Robin T. Cline, Owen White, Claire M. Fraser, Hamilton O. Smith, J. Craig Venter,

Herpesvirus Infections and Treatments

Mycoplasma genitalium with 517 genes has the smallest gene complement of any independently replicating cell so far identified. Global transposon mutagenesis was used to identify nonessential genes in an effort to learn whether the naturally occurring gene complement is a true minimal genome under laboratory growth conditions. The positions of 2209 transposon insertions in the completely sequenced genomes of M. genitalium and its close relative M. pneumoniae were determined by sequencing across the junction of the transposon and the genomic DNA. These junctions defined 1354 distinct sites of insertion that were not lethal. The analysis suggests that 265 to 350 of the 480 protein-coding genes of M. genitalium are essential under laboratory growth conditions, including about 100 genes of unknown function.